Recombinant Human Contactin-5 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Contactin-5 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 100 ng on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived human Contactin-5 protein
  Glu19-Gln1059, with a C-terminal 10-His tag
• Accession #: O94779
• N-terminal Sequence: Glu19
• Protein/Peptide Type: Recombinant Proteins
• Gene: CNTN5
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 115 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 145 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Contactin-5 Protein, CF

• CNTN5
• contactin 5
• Contactin5
• Contactin-5
• hNB-2
• HNB-2s
• MGC163491
• NB2
• NB-2
• neural adhesion molecule
• Neural recognition molecule NB-2

Background

Contactin-5 (CNTN5), also known as NB-2, is a neural adhesion molecule in the contactin family of the immunoglobulin superfamily. The contactin family comprises six members (CNTN1/F3, CNTN2/TAG-1, CNTN3/BIG-1, CNTN4/BIG-2, CNTN5/NB-2 and CNTN6/NB-3) that are characterized by the presence of six Ig-like domains, four fibronectin type III-like repeats, and a glycosylphosphatidylinositol (GPI)-anchoring domain (1, 2). The human Contactin-5 cDNA exists in two splice forms which contain an 18 amino acid (aa) signal sequence and a 28 aa C-terminal propeptide. The long isoform is a 1082 aa protein that shares 91% aa sequence identity with rat and mouse Contactin-5. The short isoform lacks the first 74 aa at the N-terminus of the long isoform. Human Contactin-5 shares 43%, 41%, 52%, 52%, and 48% aa identity with Contactins-1 ,-2 ,-3 ,-4, and -6, respectively (3). Contactin family proteins exist as membrane-bound proteins, but can also be released as soluble proteins by GPI-specific phospholipase D. The gene encoding Contactin-5 is localized to a chromosomal region associated with schizophrenia and the neuronal disorder Jacobsen syndrome (4). The highest expression of human Contactin-5 is seen in occipital lobe and amygdala, followed by cerebral cortex, frontal lobe, thalamus, and temporal lobe (4). In rat, Contactin-5 is highly expressed specifically in structures of the central auditory pathway (5). Also in rat, Contactin-5 has been shown to promote neurite outgrowth of cerebral cortical neurons in vitro (5). Deficient Contactin-5 expression in mice results in impaired neuronal activity of the central auditory system (6).

1. Kazarinova-Noyes, K. and P. Shrager (2002) Mol. Neurobiol. 26:167.
2. Denisenko-Nehrbass, N. et al. (2002) J. Physiol. Paris 96:99.
3. Ogawa, J. et al. (1996) Neurosci. Lett. 218:173.
4. Kamei, Y. et al. (2000) Genomics 69:113.
5. Ogawa, J. et al. (2001) J. Neurosci. Res. 65:100.
6. Li, H. et al. (2003) Eur. J. Neurosci. 17:929.

Bioinformatics

• Gene Symbol: CNTN5
• Uniprot:
  • O94779()