Recombinant Human CLEC9a Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human CLEC9a Fc Chimera Protein, CF Summary

Details of Functionality
Measured by flow cytometry for its ability to bind a ligand expressed in FasL-treated mouse B cells. Sancho, D. et al. (2009) Nature 458:899. At 100 ng/mL, >50% of necrotic cells bind to Recombinant Human CLEC9a.
Source
Mouse myeloma cell line, NS0-derived human CLEC9a protein
Met-Asp
Human IgG1
(Pro100-Lys330)
IEGR Human CLEC9a
(Lys57-Val241)
Accession # Q6UXN8
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
CLEC9A
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
47.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
55-65 kDa, reducing conditions
Publications
Read Publications using
6049-CL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CLEC9a Fc Chimera Protein, CF

  • CD370
  • CLEC9a
  • C-type lectin domain family 9 member A
  • C-type lectin domain family 9, member A
  • DNGR1
  • HEEE9341
  • UNQ9341

Background

CLEC9A (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily (1). Mature mouse CLEC9A consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 185 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD) (2-4). Within the ECD, human CLEC9A shares 57% aa sequence identity with mouse and rat CLEC9A. Although the CTLD of CLEC9A structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9A is expressed as a disulfide-linked homodimer of approximately 50 kDa N-glycosylated subunits (2, 4). Human CLEC9A expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16- monocytes (2-5). BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules (6). In mouse, CLEC9A is additionally expressed on plasmacytoid dendritic cells (3, 4). CLEC9A ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming (2-5, 7). Its recognition of filamentous actin in dead cells is important for triggering an immune response to necrotic cell debris (8-10). CLEC9A is also required for the presentation of viral proteins and the subsequent CTL-mediated killing of virus-infected cells (11, 12). CLEC9A signaling triggers activation of the tyrosine kinase Syk (2, 8).
  1. Kerrigan, A.M. and G.D. Brown (2010) Immunol. Rev. 234:335.
  2. Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693.
  3. Caminschi, I. et al. (2008) Blood 112:3264.
  4. Sancho, D. et al. (2008) J. Clin. Invest. 118:2098.
  5. Schreibelt, G. et al. (2012) Blood 119:2284.
  6. Dudziak, D. et al. (2007) Science 315:107.
  7. Poulin, L.F. et al. (2010) J. Exp. Med. 207:1261.
  8. Sancho, D. et al. (2009) Nature 458:899.
  9. Ahrens, S. et al. (2012) Immunity 36:635.
  10. Zhang, J.-G. et al. (2012) Immunity 36:646.
  11. Iborra, S. et al. (2012) J. Clin. Invest. 122:1628.
  12. Zelenay, S. et al. (2012) J. Clin. Invest. 122:1615.

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    Publications for CLEC9a (6049-CL)(2)

    We have publications tested in 2 confirmed species: Human, Mouse.

    We have publications tested in 2 applications: EnzAct, Flow.


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    (1)
    Flow
    (1)
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    (1)
    Mouse
    (1)
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    Bioinformatics

    Gene Symbol CLEC9A
    Entrez
    Uniprot