Recombinant Human CD52 Fc Chimera Avi-tag Protein, CF

When Recombinant Human SIGLEC-10 Fc Chimera Protein (2130-SL) is immobilized at 5.0 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CD52 Fc Chimera Avi-tag (Catalog # AVI9116) that produces 50% of the optimal binding response is 0.500-5.00 µg/mL.

2 μg/lane of Recombinant Human CD52 Fc Chimera Avi-tag Protein (Catalog # AVI9116) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-44 kDa and 80-90 kDa, respectively.

• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CD52 Fc Chimera Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human SIGLEC-10 Fc Chimera Protein (Catalog # 2130-SL) is immobilized at 5.0 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CD52 Fc Chimera Avi-tag (Catalog # AVI9116) that produces 50% of the optimal binding response is 0.500-5.00 µg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human CD52 protein
  

  Human CD52 (Gly25-Ser36) Accession # P31358.1	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
  N-terminus			C-terminus

• Accession #: P31358.1
• N-terminal Sequence: Ser24 & Gly25
• Structure / Form: Disulfide-linked homodimer
  Biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 38-44 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD52 Fc Chimera Avi-tag Protein, CF

• Cambridge pathology 1 antigen
• CAMPATH-1 antigen
• CAMPATH-1
• CD52 antigen (CAMPATH-1 antigen)
• CD52 antigen
• CD52 molecule
• CD52
• CDW52 antigen (CAMPATH-1 antigen)
• CDw52
• Epididymal secretory protein E5
• He5
• HEL-S-171mP
• Human epididymis-specific protein 5

Background

CD52, also known as CAMPATH-1 antigen, HE5, and gp20, is a cell surface glycoprotein that can be targeted to induce immune suppression by complement-mediated cell lysis (1, 2). Mature human CD52 is a 12 amino acid peptide that is tethered to the cell surface with a GPI linkage (3). It carries a large tissue-specific carbohydrate structure which increases the molecular weight of CD52 to 15-20 kDa (3-5). CD52 is expressed on lymphocytes, monocytes, monocycte-derived dendritic cells, eosinophils, and neutrophils (6-8), as well as on mature spermatozoa and epithelial cells lining the male genital tract (5, 9, 10). It protects sperm against anti-sperm antibodies by binding to C1q and inhibiting complement activation (11). CD52 ligation on CD4+ T cells induces a suppressive population of cells that release soluble CD52 which then binds to Siglec-10 (12-14). This interaction inhibits the proliferation of activated T cells and the cytotoxic function of autoimmune CD8+ T cells in type 1 diabetes (13). Our Avi-tag Biotinylated human CD52 Fc chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

1. Morris, P.J. and N.K. Russell (2006) Transplantation 81:1361.
2. Redpath, S. et al. (1998) Immunology 93:595.
3. Xia, M.Q. et al. (1991) Eur. J. Immunol. 21:1677.
4. Treumann, A. et al. (1995) J. Biol. Chem. 270:6088.
5. Schroter, S. et al. (1999) J. Biol. Chem. 274:29862.
6. Ratzinger, G. et al. (2003) Blood 101:1422.
7. Elsner, J. et al. (1996) Blood 88:4684.
8. Ambrose, L.R. et al. (2009) Blood 114:3052.
9. Focarelli, R et al. (1998) Mol. Hum. Reprod. 4:119.
10. Hale, G. et al. (1993) J. Reprod. Immunol. 23:189.
11. Hardiyanto, L. et al. (2012) J. Reprod. Immunol. 94:142.
12. Watanabe, T. et al. (2006) Clin. Immunol. 120:247.
13. Bandala-Sanchez, E. et al. (2013) Nat. Immunol. 14:741.
14. Toh, B.-H. et al. (2013) Cell. Mol. Immunol. 10:379.

Bioinformatics

• Uniprot:
  • P31358.1()