Recombinant Human CD35 Protein, CF

Images

 
There are currently no images for CD35 (5748-CD).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human CD35 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When rhCD35 is coated at 5 μg/mL, complement component C3b binds with an apparent KD <10 nM.
Source
Mouse myeloma cell line, NS0-derived human CD35 protein
Gln42-Asp1971, with a C-terminal 10-His tag and a substitution of His1208Arg
Accession #
N-terminal Sequence
No results obtained: Gln42 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
CR1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
213 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
200-220 kDa, reducing conditions
Publications
Read Publications using
5748-CD in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD35 Protein, CF

  • C3BR
  • CD35
  • complement component (3b/4b) receptor 1 (Knops blood group)
  • CR1
  • KN

Background

CD35, also known as complement receptor 1 (CR1), is an N-glycosylated member of the RCA (regulators of complement activation) family of proteins. The complement cascade plays an important role in the innate immune system through the recognition and clearance of immune complexes and foreign particles. There are four major allotypes of human CD35 which range from approximately 220 - 300 kDa (1, 2). Mature human CD35 contains a 1930 amino acid (aa) extracellular domain (ECD) with 30 tandem SCR/SUSHI repeats, a 25 aa transmembrane segment, and a 43 aa cytoplasmic tail (3). A mouse ortholog of human CD35 has not been described, although alternate splicing of mouse CD21/CR2 generates a protein with homology to some SCR repeats of human CD35 (4). A soluble form of the CD35 ECD circulates in the serum (5). Cell surface CD35 is widely expressed on hematopoietic cells (1). It cooperates with both CD21 and CR3/MAC-1 in the binding and internalization of particles and immune complexes that are opsonized with complement components C3b, C3i, and C4b (1, 6, 7). It also binds and internalizes particles opsonized with complement factor C1q or mannose binding lectin (MBL) (8, 9). CD35 protects the cell from complement-mediated lysis by serving as a cofactor for Factor I and inhibiting the C3 and C5 convertases (10). Erythrocyte-expressed CD35 mediates the hepatic clearance of complement-opsonized immune complexes (11). On lymphocytes, CD35 inhibits the activation-induced proliferation of T cells and B cells (12, 13). Mice deficient in both CD21 and CD35 exhibit normal B cell development but severely compromised germinal center development, antibody production, and establishment of protective microbial immunity (14). In mice, CD21/CD35 must additionally be present on follicular dendritic cells to mount effective humoral responses and establishment of B cell memory (15).
  1. Roozendaal, R. and M.C. Carroll (2007) Immunol. Rev. 219:157.
  2. Wong, W.W. et al. (1989) J. Exp. Med. 169:847.
  3. Klickstein, L.B. et al. (1987) J. Exp. Med. 165:1095.
  4. Kurtz, C.B. et al. (1990) J. Immunol. 144:3581.
  5. Yoon, S.H. and D.T. Fearon (1985) J. Immunol. 134:3332.
  6. Tuveson, D.A. et al. (1991) J. Exp. Med. 173:1083.
  7. Sutterwala, F.S. et al. (1996) J. Leukoc. Biol. 59:883.
  8. Klickstein, L.B. et al. (1997) Immunity 7:345.
  9. Ghiran, I. et al. (2000) J. Exp. Med. 192:1797.
  10. Iida, K. and V. Nussenzweig (1981) J. Exp. Med. 153:1138.
  11. Cornacoff, J.B. et al. (1983) J. Clin. Invest. 71:236.
  12. Wagner, C. et al. (2006) Mol. Immunol. 43:643.
  13. Jozsi, M. et al. (2002) J. Immunol. 168:2782.
  14. Haas, K.M. et al. (2002) Immunity 17:713.
  15. Rossbacher, J. et al. (2006) Eur. J. Immunol. 36:2384.

Customers Who Viewed This Item Also Viewed...

NBP2-38895
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-540
Species: Mu
Applications: Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
MAB5376
Species: Mu
Applications: WB, Flow, CyTOF-ready
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
NB110-89474
Species: Hu, Mu, Rt, Bv, ChHa, Pm, RM
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ISH, Dual ISH-IHC, Single-Cell Western
MAB2005
Species: Hu
Applications: WB, Flow, CyTOF-ready
AF1730
Species: Hu
Applications: Flow, AdBlk, CyTOF-ready, ICC
NB500-330
Species: Hu
Applications: Flow, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready
AF1008
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
AF4779
Species: Hu
Applications: WB, IHC
NB200-541
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
NBP1-90214
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
5748-CD
Species: Hu
Applications: Binding Activity

Publications for CD35 (5748-CD)(3)

Reviews for CD35 (5748-CD) (0)

There are no reviews for CD35 (5748-CD). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CD35 (5748-CD) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional CD35 Products

Bioinformatics Tool for CD35 (5748-CD)

Discover related pathways, diseases and genes to CD35 (5748-CD). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD35 (5748-CD)

Discover more about diseases related to CD35 (5748-CD).
 

Pathways for CD35 (5748-CD)

View related products by pathway.

PTMs for CD35 (5748-CD)

Learn more about PTMs related to CD35 (5748-CD).

Blogs on CD35.

How to identify B cell subsets using flow cytometry
By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

CD11b Antibody
NB110-89474

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human CD35 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol CR1
Uniprot