Recombinant Human BTN2A2/Butyrophilin 2A2 Fc Protein, CF

Recombinant Human BTN2A2/Butyrophilin 2A2 Fc Chimera (Catalog # 8918-BT) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.1‑0.5 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human BTN2A2/Butyrophilin 2A2 Fc Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 0.1-0.5 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human BTN2A2/Butyrophilin 2A2 protein
  

  Human BTN2A2 (Gln33-Val237) Accession # Q8WVV5	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q8WVV5
• N-terminal Sequence: No result obtained. Gln33 inferred from enzymatic pyroglutamate treatment revealing Phe34
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: BTN2A2
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BTN2A2/Butyrophilin 2A2 Fc Protein, CF

• BTF2
• BTF2BT2.2butyrophilin 2
• BTN2
• BTN2A2
• Butyrophilin 2
• butyrophilin subfamily 2 member A2
• butyrophilin, subfamily 2, member A2
• FLJ41908

Background

Butyrophilin 2A2 (BTN2A2) is a widely expressed 65 kDa type I transmembrane glycoprotein that functions as a negative regulator of immune responses (1). Mature human Butyrophilin 2A2 consisits of a 233 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 237 aa cytoplasmic domain. Within the Ig-like domains, human Butyrophilin 2A2 shares 70% aa sequence identity with mouse and rat Butyrophilin 2A2. Alternative splicing generates additional isoforms of human Butyrophilin 2A2 that lack the first, second, or both Ig-like domains as well as isoforms with substitutions and deletions in the cytoplasmic region. Within the immune system, Butyrophilin 2A2 is expressed on thymic epithelial cells, naïve B cells, splenic NK cells, dendritic cells, and peritoneal macrophages and is up-regulated with cell activation (2, 3). Butyrophilin 2A2 inhibits T cell proliferation and activation and enhances the development of FoxP3⁺ regulatory T cells (2, 3). Its up-regulation in the hippocampus is associated with schizophrenia (4).

1. Arnett, H.A. and J.L. Viney (2014) Nat. Rev. Immunol. 14:559.
2. Smith, I.A. et al. (2010) J. Immunol. 184:3514.
3. Ammann, J.U. et al. (2013) J. Immunol. 190:5030.
4. Sinkus, M.L. et al. (2013) Brain Behav. Immun. 32:51.

Bioinformatics

• Gene Symbol: BTN2A2
• Uniprot:
  • Q8WVV5(Human)