Recombinant Human Annexin A2 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human Annexin A2 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: E. coli-derived human Annexin A2 protein
  Ser2-Asp339, with a C-terminal 6-His tag
• Accession #: P07355-1
• N-terminal Sequence: Ser2
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 38 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Annexin A2 Protein, CF

• Annexin A2
• Annexin II
• annexin-2
• ANX2
• ANX2L4
• ANX2L4LPC2
• ANXA2
• CAL1H
• Calpactin I heavy chain
• calpactin I heavy polypeptide
• Calpactin-1 heavy chain
• chromobindin 8
• chromobindin-8
• LIP2
• LIP2PAP-IV
• Lipocortin II
• Lipocortin-2
• LPC2D
• LPC2DP36
• p36
• PAP-IV
• Placental anticoagulant protein IV
• Protein I

Background

Annexin A2 (ANXA2), also known as Annexin II and Lipocortin II (LPC2), is a 39 kDa member of the Annexin family. Annexins are calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain is comprised of four 60-70 aa annexin repeats which form a tightly packed disc known as the annexin domain. Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (1). Annexin A2 is heavily involved in membrane organization and traffic (2). Annexin A2 also functions as an autocrine factor to enhance osteoclast formation and bone resorption and is a major cellular substrate of the tyrosine kinase encoded by the SRC oncogene (3, 4). Human Annexin A2 shares 97% identity with mouse and rat Annexin A2.

1. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
2. Hayes, M. et al. (2004). Traffic. 5:571.
3. Siever, D. A., et al. (1997). ‎Int. J. Biochem. Cell Biol. 29:1219.
4. Thiel, C. A. et al. (1992). J. Cell Sci. 103:733.

Bioinformatics

• Uniprot:
  • P07355-1()