Recombinant Human AMIGO2 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human AMIGO2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED₅₀ for this effect is 0.5-2.0 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human AMIGO2 protein
  

  Human AMIGO2 (Gly39-His393) Accession # Q86SJ2	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q86SJ2
• N-terminal Sequence: Gly39 & Val40
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: AMIGO2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 66.9 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90-110 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human AMIGO2 Fc Chimera Protein, CF

• adhesion molecule with Ig-like domain 2
• ALI1amphoterin induced gene 2
• alivin 1
• Alivin-1
• AMIGO2
• AMIGO-2
• DEGAamphoterin-induced protein 2
• differentially expressed in gastric adenocarcinoma
• Differentially expressed in gastric adenocarcinomas
• transmembrane protein AMIGO2

Background

AMIGO2 (amphoterin‑induced gene and ORF 2), also known as DEGA and Alivin‑1, is an approximately 65 kDa transmembrane cell adhesion protein. It belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1, 2). Mature human AMIGO2 consists of a 359 amino acid (aa) extracellular domain (ECD) with six LRRs flanked by single LRRNT and LRRCT domains, followed by one immunoglobulin-like domain, a 21 aa transmembrane segment, and a 103 aa cytoplasmic domain (3‑5). Within the ECD, human AMIGO2 shares 89% and 87% aa sequence identity with mouse and rat AMIGO2, respectively. AMIGO2 forms homodimers as well as heterodimers with the related AMIGO and AMIGO3 molecules (3, 6). Within the nervous system, AMIGO2 is transcribed in the cerebrum, hypothalamus, olfactory bulb, retina, hippocampus (pyramidal cells), and cerebellum (granule neurons and Purkinje cells) (3, 4, 7). It is also expressed in the liver, lung, testis, spleen, and small intestine (3, 4). AMIGO2 supports neuron survival, and is down‑regulated following pro‑apoptotic stimulation (4). Its expression can be up‑regulated or down‑regulated in a variety of cancers, and anti‑sense knockdown of AMIGO2 interferes with tumor cell adhesion to collagen as well as in vivo tumorigenicity (5).

1. de Wit, J. et al. (2011) Annu. Rev. Cell Dev. Biol. 27:697.
2. Chen, Y. et al. (2006) Brain Res. Rev. 51:265.
3. Kuja-Panula, J. et al. (2003) J. Cell Biol. 160:963.
4. Ono, T. et al. (2003) J. Neurosci. 23:5887.
5. Rabenau, K.E. et al. (2004) Oncogene 23:5056.
6. Kajander, T. et al. (2011) J. Mol. Biol. 413:1001.
7. Laeremans, A. et al. (2013) Brain Struct. Funct. 218:123.

Bioinformatics

• Gene Symbol: AMIGO2
• Uniprot:
  • Q86SJ2()