Recombinant Human AMIGO Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human AMIGO Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance survival of E16-E18 rat embryonic cortical neurons.

  The ED₅₀ for this effect is 0.6-2.4 μg/mL.

  Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth is observed between 1.25-10 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human AMIGO protein
  Gly28-His367, with a C-terminal 6-His tag
• Accession #: Q86WK6
• N-terminal Sequence: Starts at Gly28
• Protein/Peptide Type: Recombinant Proteins
• Gene: AMIGO1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 39.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human AMIGO Protein, CF

• adhesion molecule with Ig-like domain 1
• ali2
• Alivin-2
• AMIGO
• AMIGO1
• AMIGO-1
• AMIGOamphoterin-induced protein 1
• amphoterin-induced gene and ORF
• KIAA1163

Background

AMIGO (amphoterin-induced gene and ORF) is an approximately 75 kDa transmembrane cell adhesion protein. It belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1). Mature human AMIGO consists of a 345 amino acid (aa) extracellular domain (ECD) with six tandem LRRs flanked by LRR N- and C‑terminal domains, one immunoglobulin‑like domain, a 21 aa transmembrane segment, and a 100 aa cytoplasmic domain (2). Within the ECD, human AMIGO shares 86% aa sequence identity with mouse and rat AMIGO. AMIGO is an N‑glycosylated protein that forms homodimers by association between LRR regions (3, 4). It interacts homophilically as well as heterophilically with the related AMIGO2 and AMIGO3 proteins (2). AMIGO is expressed along developing and mature neuronal fiber tracts on neurons, astrocytes, and oligodendrocytes (2, 4). It promotes the extension and branching of hippocampal and cortical neurons (2, 4). It also supports neuronal survival following apoptotic stimulation (4). AMIGO directly associates with the potassium channel Kv2.1 and enhances ion conductance through the channel (5). Kv2.1 localization and activation is regulated by phosphorylation, but phosphorylation status does not alter its interaction with AMIGO (5, 6).

1. de Wit, J. et al. (2011) Annu. Rev. Cell Dev. Biol. 27:697.
2. Kuja-Panula, J. et al. (2003) J. Cell Biol. 160:963.
3. Kajander, T. et al. (2011) J. Mol. Biol. 413:1001.
4. Chen, Y. et al. (2012) J. Cell. Physiol. 227:2217.
5. Peltola, M.A. et al. (2011) EMBO Rep. 12:1293.
6. Park, K.-S. et al. (2006) Science 313:976.

Bioinformatics

• Gene Symbol: AMIGO1
• Uniprot:
  • Q86WK6()