Recombinant Cynomolgus Monkey IL-18 BPa His-tag Protein, CF

Recombinant Cynomolgus Monkey IL‑18 BPa inhibits Recombinant Human IL-18-induced IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 0.015-0.075 μg/mL.

2 μg/lane of Recombinant Cynomolgus Monkey IL‑18 BPa was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 54-60 kDa.

• Reactivity: Pm-Cm
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus Monkey IL-18 BPa His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the IL-18-induced response of KG-1 human acute myelogenous leukemia cells. The ED 50 for this effect is 0.015-0.075 μg/mL
• Source: Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey IL-18 BPa protein Thr28-Pro207, with a C-terminal 6-His tag
• Accession #: XP_015290916.1
• N-terminal Sequence: Thr28
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 54-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus Monkey IL-18 BPa His-tag Protein, CF

• IL18 BPa
• IL-18 BPa
• IL18BP
• IL-18BP
• IL18BPa
• interleukin 18 binding protein
• interleukin-18-binding protein
• MC51L-53L-54L homolog gene product
• tadekinig-alfa

Background

Cynomolgus Interleukin 18 binding protein (IL-18 BP) is a 20 kDa secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity (1). IL-18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. Human IL-18BP encodes for at least four isoforms by alternative splicing. The IL-18 BP isoform a and c each contains one immunoglobulin (Ig)-like C2-type domain while isoform b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins (2). Cynomolgus IL-18BPa shares 83% sequence identity with human and 62% with mouse IL-18BPa, respectively. Several poxviruses also encode proteins with sequence similarity to IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly upregulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP (3).

1. Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
2. Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
3. Calderara, S. et al. (2001) Virology 279:22.

Bioinformatics

• Uniprot:
  • XP_015290916.1()