REC8 Overexpression Lysate (Native)

Images

 
Western Blot: REC8 Overexpression Lysate (Adult Normal) [NBL1-15263] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for REC8.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

REC8 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC219012 and based on accession number NM_001048205. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens REC8 homolog (yeast) (REC8), transcript variant 2, mRNA.
Gene
REC8

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
62.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for REC8

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for REC8 Overexpression Lysate (Native)

  • Cohesin Rec8p
  • cohesion rec8p
  • HR21spB
  • meiotic recombination and sister chromatid cohesion phosphoprotein of therad21p family
  • meiotic recombination protein REC8 homolog
  • meiotic recombination protein REC8-like 1
  • MGC950
  • REC8 homolog (yeast)
  • REC8L1human homolog of rad21, S. pombe
  • REC8-like 1 (yeast)
  • REC8-like 1
  • Rec8p
  • recombination and sister chromatid cohesion protein homolog

Background

This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for REC8 Lysate (NBL1-15263) (0)

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Video Protocols

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Bioinformatics

Gene Symbol REC8