REC8 Antibody

Product Discontinued

REC8 Antibody Summary

• Immunogen: This antibody was made against a protein fragment from the C Terminus Region
• Epitope: MRTEEELENVRRRQKSSLGVQFMRTDDLEEDTRRNRLFEDEERTRDAREDELFFYSSGSLLPNNRLNIHKELLNEAEARYPEWVNFNEFTADHDRKKAAT
• Specificity: This product is specific for C. elegans REC8.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: REC8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry
• Application Notes: This antibody works in Immunofluorescence (PMID: 22018922). The concentration for this product is lot dependent. Check product vial for current lot concentration. Typically concentration ranges from 0.5 to 1.5 mg/ml. Use in Immunohistochemistry reported in scientific literature (PMID 24367695)

Reactivity Notes

Caenorhabditis elegans

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

This product was created from the ModEncode Project, a part of the NHGRI, and is sold by SDIX and Novus Biologicals. These C. elegans antibodies were generated in the labs of Jason Lieb, Susan Strome, Julie Ahringer, Arshad Desai, and Abby Dernburg.

Alternate Names for REC8 Antibody

• Cohesin Rec8p
• cohesion rec8p
• HR21spB
• meiotic recombination and sister chromatid cohesion phosphoprotein of therad21p family
• meiotic recombination protein REC8 homolog
• meiotic recombination protein REC8-like 1
• MGC950
• REC8 homolog (yeast)
• REC8L1human homolog of rad21, S. pombe
• REC8-like 1 (yeast)
• REC8-like 1
• Rec8p
• recombination and sister chromatid cohesion protein homolog

Background

This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for REC8 Antibody (29470002)(12)

Publications using 29470002

• Castellano-Pozo M, Pacheco S, Sioutas G et al. Surveillance of cohesin-supported chromosome structure controls meiotic progression Nat Commun 2020-08-28 [PMID: 32859945]
• Wang X, Ellenbecker M, Hickey B et al. Antagonistic control of Caenorhabditis elegans germline stem cell proliferation and differentiation by PUF proteins FBF-1 and FBF-2 Elife 2020-08-17 [PMID: 32804074] (IF/IHC, C. elegans)
• Ellenbecker M, Osterli E, Wang X et al. Dynein Light Chain DLC-1 Facilitates the Function of the Germline Cell Fate Regulator GLD-1 in Caenorhabditis elegans Genetics 2018-12-03 [PMID: 30509955] (ICC/IF, C. elegans)
• Hernandez M. R, Davis M. B, et al. Condensin I protects meiotic cohesin from WAPL-1 mediated removal. PLoS Genet 2018-05-01 [PMID: 29768402] (ICC/IF, C. elegans)
• Dawson JA, Methven-Kelley C, Davis GM. atz-1 influences meiosis to maintain germline chromosomal stability in Caenorhabditis elegans Cell Biol. Int. 2017-07-11 [PMID: 28696027] (IF/IHC, C. elegans)
• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Crawley O, Barroso C, Testori S et al. Cohesin-interacting protein WAPL-1 regulates meiotic chromosome structure and cohesion by antagonizing specific cohesin complexes. Elife. 2016-02-04 [PMID: 26841696] (ICC/IF, C. elegans)
• Severson AF, Meyer BJ. Divergent kleisin subunits of cohesin specify mechanisms to tether and release meiotic chromosomes. Elife. 2014-08-29 [PMID: 25171895]
• Ko S, Kawasaki I, Shim YH. PAB-1, a Caenorhabditis elegans Poly(A)-Binding Protein, Regulates mRNA Metabolism in germline by Interacting with CGH-1 and CAR-1. PLoS One 2013-12-19 [PMID: 24367695] (IF/IHC, ICC/IF, C. elegans)
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.
• Xu J, Sun X, Jing Y et al. MRG-1 is required for genomic integrity in Caenorhabditis elegans germ cells. Cell Res 2012-05-01 [PMID: 22212480]
• Harper NC, Rillo R, Jover-Gil S et al. Pairing Centers Recruit a Polo-like Kinase to Orchestrate Meiotic Chromosome Dynamics in C. elegans Developmental Cell. 2011-10-19 [PMID: 22018922]

Bioinformatics

• Gene Symbol: REC8
• Uniprot:
  • W02A2.6(C. elegans)