RDH5 Antibody

Western Blot: RDH5 Antibody [NBP2-93754] - Analysis of extracts of various cell lines, using RDH5 . Exposure time: 90s.

Immunohistochemistry: RDH5 Antibody [NBP2-93754] - Analysis of rat retina using RDH5 . Blue: DAPI for nuclear staining.

Immunohistochemistry: RDH5 Antibody [NBP2-93754] - Analysis of mouse retina using RDH5 . Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RDH5 Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-318 of human RDH5 (NP_002896.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RDH5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RDH5 Antibody

• 11-cis RDH
• 11-cis retinol dehydrogenase
• 9,9-cis-retinol specific dehydrogenase
• EC 1.1.1
• EC 1.1.1.105
• FLJ39337
• FLJ97089
• HSD17B
• RDH1
• retinol dehydrogenase 1
• retinol dehydrogenase 5 (11-cis and 9-cis)
• retinol dehydrogenase 5 (11-cis/9-cis)
• SDR9C5
• short chain dehydrogenase/reductase family 9C, member 5

Background

RDH5, also known as 11-cis retinol dehydrogenase, is a 35 kDa 318 amino acid protein located in the membrane. RDH5 is involved with the catalyzation in the last step of the biosynthesis of 11-cis retinaldehyde. Current research on RDH5 is being conducted in relation to night blindness, liver disease, adenomatous polyposis coli, cone dystrophy, and macular dystrophy. RDH5 is linked to the vitellogenesis, regeneration, oocyte growth and metaphase pathways where it interacts with DGAT1, ALDH1A2, ALDH1A1, RLBP1 and RGR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RDH5