RCC1 Antibody (3D11)

Western Blot: RCC1 Antibody (3D11) [NBP1-97543] - Analysis of RanGEF probed with RanGEF (RCC1), mAb (3D11).

Product Discontinued

RCC1 Antibody (3D11) Summary

• Immunogen: Recombinant human RanGEF.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RCC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunoprecipitation 2 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: This antibody is useful in Immunocytochemistry, Immunoprecipitation and Western Blot (Detects a band of ~45kDa).

Reactivity Notes

Recognizes Human, Mouse and Rat RanGEF. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 1.0% Sucrose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with 0.1 ml sterilized distilled water to a final concentration of 1.0 mg/ml.

Alternate Names for RCC1 Antibody (3D11)

• Cell cycle regulatory protein
• CHC1RCC1-I
• chromosome condensation 1
• Chromosome condensation protein 1
• guanine nucleotide-releasing protein
• regulator of chromosome condensation 1
• regulator of chromosome condensation
• SNHG3-RCC1 readthrough transcript
• SNHG3-RCC1

Background

RanGEF, also known as regulator of chromosome condensation (RCC1), is an abundant, highly conserved chromatin-associated 45 kDa protein. RanGEF is involved in the regulation of the onset of chromosome condensation in the S phase, and is the principle mammalian guanine nucleotide exchange factor (GEF) for the nuclear G protein Ran. RanGEF plays important roles in RNA transcription and processing, DNA replication, nuclear pore transport function, and cell cycle regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RCC1