RCC1 Antibody (1C1) - Azide and BSA Free

Western Blot: RCC1 Antibody (1C1) [H00001104-M02] - Analysis of RCC1 expression in transfected 293T cell line by RCC1 monoclonal antibody (M02), clone 1C1.Lane 1: RCC1 transfected lysate(45 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: RCC1 Antibody (1C1) [H00001104-M02] - Analysis of monoclonal antibody to RCC1 on HeLa cell . Antibody concentration 15 ug/ml.

Immunoprecipitation: RCC1 Antibody (1C1) [H00001104-M02] - Analysis of RCC1 transfected lysate using anti-RCC1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RCC1 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: RCC1 Antibody (1C1) [H00001104-M02] - Detection limit for recombinant GST tagged RCC1 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 1C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RCC1 Antibody (1C1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RCC1 (AAH07300, 312 a.a. ~ 421 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQS
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RCC1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot
• Application Notes: It has been used for ELISA and WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RCC1 Antibody (1C1) - Azide and BSA Free

• Cell cycle regulatory protein
• CHC1RCC1-I
• chromosome condensation 1
• Chromosome condensation protein 1
• guanine nucleotide-releasing protein
• regulator of chromosome condensation 1
• regulator of chromosome condensation
• SNHG3-RCC1 readthrough transcript
• SNHG3-RCC1

Background

RCC1 (regulator of chromosome condensation) is a 45 kD nuclear protein containing RCC1 repeats.This widely expressed protein associates with chromosomes and acts as a guanine nucleotide exchange factor (GEF) protein for Ras-related GTPase Ran. RCC1 is thought to maintain the Ran GTP gradient required for nucleocytoplasmic transport and spindle assembly across the interphase nuclear envelope and on condensed mitotic chromosomes. RCC1 regulates cell cycle and also plays a role in nuclear pore transport. Defects in RCC1 results in abnormal chromosome segregation and genomic instability. RCC1 is transcriptionally activated by Myc through E-box elements. RCC1 binds to chromatin in a binary complex with Ran. The Poly6164 antibody recognizes the N-terminal region of the human and mouse RCC1 protein and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RCC1
• Uniprot:
  • AAH07300.1(Human)