RCC1 Antibody (1C1)

Images

 
Western Blot: RCC1 Antibody (1C1) [H00001104-M02] - Analysis of RCC1 expression in transfected 293T cell line by RCC1 monoclonal antibody (M02), clone 1C1.Lane 1: RCC1 transfected lysate(45 KDa).Lane 2: Non-transfected ...read more
Immunocytochemistry/ Immunofluorescence: RCC1 Antibody (1C1) [H00001104-M02] - Analysis of monoclonal antibody to RCC1 on HeLa cell. Antibody concentration 15 ug/ml.
Immunoprecipitation: RCC1 Antibody (1C1) [H00001104-M02] - Analysis of RCC1 transfected lysate using anti-RCC1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RCC1 MaxPab rabbit polyclonal ...read more
Sandwich ELISA: RCC1 Antibody (1C1) [H00001104-M02] - Detection limit for recombinant GST tagged RCC1 is 0.3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
1C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RCC1 Antibody (1C1) Summary

Immunogen
RCC1 (AAH07300 312 a.a. - 421 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQS
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RCC1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Application Notes
It has been used for ELISA and WB.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RCC1 Antibody (1C1)

  • Cell cycle regulatory protein
  • CHC1RCC1-I
  • chromosome condensation 1
  • Chromosome condensation protein 1
  • guanine nucleotide-releasing protein
  • regulator of chromosome condensation 1
  • regulator of chromosome condensation
  • SNHG3-RCC1 readthrough transcript
  • SNHG3-RCC1

Background

RCC1 (regulator of chromosome condensation) is a 45 kD nuclear protein containing RCC1 repeats.This widely expressed protein associates with chromosomes and acts as a guanine nucleotide exchange factor (GEF) protein for Ras-related GTPase Ran. RCC1 is thought to maintain the Ran GTP gradient required for nucleocytoplasmic transport and spindle assembly across the interphase nuclear envelope and on condensed mitotic chromosomes. RCC1 regulates cell cycle and also plays a role in nuclear pore transport. Defects in RCC1 results in abnormal chromosome segregation and genomic instability. RCC1 is transcriptionally activated by Myc through E-box elements. RCC1 binds to chromatin in a binary complex with Ran. The Poly6164 antibody recognizes the N-terminal region of the human and mouse RCC1 protein and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on RCC1.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

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Bioinformatics

Gene Symbol RCC1
Uniprot