RBM15 Antibody

Western Blot: RBM15 Antibody [21390002] - Samples: Lane 1: Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 / Lane 2: RT-4 / Lane 3: U-251MG sp / Lane 4: Human Plasma / Lane 5: Liver / Lane 6: Tonsil; Target weight [kDa]: 107, 106, 3 (splice variants)

Immunohistochemistry: RBM15 Antibody [21390002] - A majority of normal and malignant tissues showed moderate to strong nuclear and moderate cytoplasmic staining. In normal tissues, strongest positivity was found in gastro-intestinal glandular cells and in neuronal cells of the CNS. Prostate, bile duct cells and ovarian stromal cells were negative. Malignant tissues stained similarly, with strongest immunoreactivity found in colo-rectal cancers and malignant gliomas. Other tumors generally displayed moderate nuclear and cytoplasmic staining. Most prostate cancers were negative. Image and statement courtesy of the Human Protein Atlas (HPA).

ELISA: RBM15 Antibody [21390002] - Affinity Purified

Product Discontinued

RBM15 Antibody Summary

• Immunogen: This antibody is specific for the Middle Region of the target protein.
• Specificity: This product is specific for Human RBM15.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBM15
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for RBM15 Antibody

• FLJ12479
• one twenty two protein
• one twenty-two
• One-twenty two protein 1
• putative RNA-binding protein 15
• RNA binding motif protein 15
• RNA-binding motif protein 15
• SPEN

Background

Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RBM15 Antibody (21390002)(3)

Publications using 21390002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Kannan K, Coarfa C, Rajapakshe K et al. CDKN2D-WDFY2 Is a Cancer-Specific Fusion Gene Recurrent in High-Grade Serous Ovarian Carcinoma. PLoS Genet. 2014-03-01 [PMID: 24675677] (WB, Human)
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: RBM15
• Uniprot:
  • NM_022768(Human)