RBCK1 Antibody

Images

 
Western Blot: RBCK1 Antibody [NBP2-27105] - Analysis of RBCK1 using RBCK1 antibody. A549 cell lysate in the 1) pre immune sera and 2) abesence of immunizing peptide and 3) 3T3 cell lysate probed with 2 ug/ml of RBCK1 ...read more
Immunocytochemistry/ Immunofluorescence: RBCK1 Antibody [NBP2-27105] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, Pm, RMSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

RBCK1 Antibody Summary

Immunogen
A portion of amino acids 150-200 from human RBCk1 was used as the immunogen for this antibody.
Predicted Species
Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RBCK1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 5-10 ug/ml
  • Western Blot 2-4ug/ml

Reactivity Notes

Orangutan, Chimpanzee (100%), New world monkey (89%), Bovine, Guinea Pig, Mole Rat (83%), Canine, Elephant, Rabbit (78%), Panda, Oppossum (67%), Rat (61%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for RBCK1 Antibody

  • C20orf18
  • EC 6.3.2.-
  • HBV associated factor 4
  • HBV-associated factor 4
  • Heme-oxidized IRP2 ubiquitin ligase 1
  • Hepatitis B virus X-associated protein 4
  • HOIL-1
  • RanBP-type and C3HC4-type zinc finger containing 1
  • ranBP-type and C3HC4-type zinc finger-containing protein 1
  • RBCC protein interacting with PKC1
  • RBCK2
  • RNF54RING finger protein 54
  • UBCE7IP3HOIL1
  • ubiquitin conjugating enzyme 7 interacting protein 3
  • Ubiquitin-conjugating enzyme 7-interacting protein 3
  • XAP3
  • XAP4chromosome 20 open reading frame 18
  • ZRANB4

Background

RBCk1, also known as HOIL-1L, acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex that contains a zf-RanBP (Zn-finger in Ran binding protein) and a zf-C3HC4 (RING finger) domain. RBCk1 catalyzes the ubiquitination and degradation of IRF3. Both RBCk1 and IRF3 are activated by viral infections and IRF3 is a prerequisite trigger for induction of type I Interferons (1). RBCk1 also negatively regulates TNF and IL1 induced NF-fUB activation (2). Thus it appears RBCk1 and IRF3 interaction represents a controlling antiviral regulatory channel but also that RBCk1 and ubiquitination pathways (3, 4) also are related to NF-kB and its role as an anti-viral response pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RBCK1
Uniprot