RBCK1 Antibody - BSA Free

Western Blot: RBCK1 Antibody [NBP2-27105] - Analysis of RBCK1 using RBCK1 antibody. A549 cell lysate in the 1) pre immune sera and 2) abesence of immunizing peptide and 3) 3T3 cell lysate probed with 2 ug/ml of RBCK1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunocytochemistry/Immunofluorescence: RBCK1 Antibody [NBP2-27105] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-RBCK1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• Reactivity: Hu, Mu, Pm, RM
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RBCK1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RBCK1 Antibody - BSA Free (NBP2-27105) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-RBCK1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 150-200 from human RBCk1 was used as the immunogen for this antibody.
• Predicted Species: Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBCK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5-10 ug/ml
  • Western Blot 2-4ug/ml

Reactivity Notes

Orangutan, Chimpanzee (100%), New world monkey (89%), Bovine, Guinea Pig, Mole Rat (83%), Canine, Elephant, Rabbit (78%), Panda, Oppossum (67%), Rat (61%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for RBCK1 Antibody - BSA Free

• C20orf18
• EC 6.3.2.-
• HBV associated factor 4
• HBV-associated factor 4
• Heme-oxidized IRP2 ubiquitin ligase 1
• Hepatitis B virus X-associated protein 4
• HOIL-1
• RanBP-type and C3HC4-type zinc finger containing 1
• ranBP-type and C3HC4-type zinc finger-containing protein 1
• RBCC protein interacting with PKC1
• RBCK2
• RNF54RING finger protein 54
• UBCE7IP3HOIL1
• ubiquitin conjugating enzyme 7 interacting protein 3
• Ubiquitin-conjugating enzyme 7-interacting protein 3
• XAP3
• XAP4chromosome 20 open reading frame 18
• ZRANB4

Background

RBCk1, also known as HOIL-1L, acts as an E3 ubiquitin-protein ligase, or as part of an E3 complex that contains a zf-RanBP (Zn-finger in Ran binding protein) and a zf-C3HC4 (RING finger) domain. RBCk1 catalyzes the ubiquitination and degradation of IRF3. Both RBCk1 and IRF3 are activated by viral infections and IRF3 is a prerequisite trigger for induction of type I Interferons (1). RBCk1 also negatively regulates TNF and IL1 induced NF-fUB activation (2). Thus it appears RBCk1 and IRF3 interaction represents a controlling antiviral regulatory channel but also that RBCk1 and ubiquitination pathways (3, 4) also are related to NF-kB and its role as an anti-viral response pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RBCK1 Antibody (NBP2-27105)(1)

Publication using NBP2-27105

• Damgaard R, Walker J, Marco-Csanova P The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity Cell, 2016-08-11;0(0):. 2016-08-11 [PMID: 27523608]

Bioinformatics

• Gene Symbol: RBCK1
• Uniprot:
  • Q9BYM8(Human)