RBBP4/RbAp48 Antibody

Images

 
Western Blot: RBBP4/RbAp48 Antibody [NB100-60399] - Whole cell lysate from HeLa (5, 15, and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: NB100-60399 used ...read more
Immunohistochemistry: RBBP4/RbAp48 Antibody [NB100-60399] - Sample: FFPE section of human Ewing sarcoma. Antibody: Affinity purified rabbit anti- RBBP4/RbAp48 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody [NB100-60399] - FFPE section of human bladder cell carcinoma. Antibody used at a dilution of 1:500.
Proximity Ligation Assay: RBBP4/RbAp48 Antibody [NB100-60399] - The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

RBBP4/RbAp48 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human retinoblastoma binding protein 4 (retinoblastoma-binding protein p48) using the numbering given in entry CAA52321.1 (GeneID 5928).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RBBP4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg
  • Proximity Ligation Assay 1:200-1:4000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
RBBP4/RbAp48 Lysate (NBL1-15185)
Publications
Read Publications using
NB100-60399 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RBBP4/RbAp48 Antibody

  • CAF-1 p48
  • CAF-1 subunit C
  • CAF-1C
  • CAF-I p48
  • Chromatin assembly factor 1 subunit C
  • Chromatin assembly factor I p48 subunit
  • chromatin assembly factor/CAF-1 p48 subunit
  • histone-binding protein RBBP4
  • MSI1 protein homolog
  • Nucleosome-remodeling factor subunit RBAP48
  • NURF55
  • RBAP48
  • RBBP4
  • RBBP-4
  • retinoblastoma binding protein 4
  • Retinoblastoma-binding protein 4CAF-I 48 kDa subunit
  • Retinoblastoma-binding protein p48

Background

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RBBP4/RbAp48 Antibody (NB100-60399)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RBBP4
Entrez