RbAp46 Antibody (OTI5A4)

Western Blot: RbAp46 Antibody (5A4) [NBP2-01308] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RbAp46 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RbAp46.

Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] Staining of paraffin-embedded Human prostate tissue using anti-RbAp46 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RbAp46 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RbAp46 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RbAp46 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RbAp46 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Human endometrium tissue using anti-RbAp46 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Human Kidney tissue using anti-RbAp46 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RbAp46 Antibody (5A4) [NBP2-01308] - Staining of paraffin-embedded Human pancreas tissue using anti-RbAp46 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI5A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.84 mg/ml

RbAp46 Antibody (OTI5A4) Summary

• Immunogen: Full length human recombinnat protein of RBBP7(NP_002884) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBBP7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500
• Theoretical MW: 47.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.84 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RbAp46 Antibody (OTI5A4)

• G1/S transition control protein-binding protein RbAp46
• Histone acetyltransferase type B subunit 2
• histone-binding protein RBBP7
• MGC138867
• Nucleosome-remodeling factor subunit RBAP46
• RbAp46
• RBBP-7
• retinoblastoma binding protein 7
• Retinoblastoma-binding protein 7MGC138868
• Retinoblastoma-binding protein p46
• retinoblastoma-binding protein RbAp46

Background

This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RbAp46 Antibody (NBP2-01308)(1)

Publication using NBP2-01308

Bioinformatics

• Gene Symbol: RBBP7