RB1 [p Thr821] Antibody

Product Discontinued

RB1 [p Thr821] Antibody Summary

• Immunogen: Synthetic peptide (Human) derived from a region of human Rb that contains threonine 821 (based on Swiss Protein database, accession number P06400). The sequence is conserved in human, mouse and rat.
• Modification: p Thr821
• Localization: Nuclear
• Specificity: Rb [Thr821]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: WB: Use at a concentration of 0.25 - 1.0 ug/ml. Predicted molecular weight: 106 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human. Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Dulbecco's PBS (pH 7.3), 1.0 mg/ml and 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RB1 [p Thr821] Antibody

• OSRC
• osteosarcoma
• p105-Rb
• pp110
• pRb
• RB
• RB1
• retinoblastoma 1
• retinoblastoma suspectibility protein
• retinoblastoma-associated protein

Background

Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RB1
• Entrez:
  • 5925(Human)