RB1 Antibody

Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-48953] - Staining of human Retina shows strong nuclear positivity in photoreceptor cells, cells in inner nuclear layer and ganglion cells.

Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-48953] - Staining of human Cervix, uterine shows strong nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-48953] - Staining of human Lymph node shows strong nuclear positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-48953] - Staining of human Placenta shows strong nuclear positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-48953] - Staining of human Skin shows strong nuclear positivity in epidermal cells.

Product Discontinued

RB1 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: PLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Mouse (89%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RB1 Antibody

• OSRC
• osteosarcoma
• p105-Rb
• pp110
• pRb
• RB
• RB1
• retinoblastoma 1
• retinoblastoma suspectibility protein
• retinoblastoma-associated protein

Background

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of ts function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RB1