RB1 Antibody

Product Discontinued

RB1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 741-790 of Human Rb.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Retinoblastoma 1 (T774) pAb in extracts from K562 cells.
  Immunohistochemistry (IHC) analyzes of Retinoblastoma 1 (T774) pAb in paraffin-embedded human breast carcinoma tissue.
• Theoretical MW: 106 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RB1 Antibody

• OSRC
• osteosarcoma
• p105-Rb
• pp110
• pRb
• RB
• RB1
• retinoblastoma 1
• retinoblastoma suspectibility protein
• retinoblastoma-associated protein

Background

Pediatric cancer retinoblastoma and the formation of other human tumors can be attributed to mutations in the retinoblastoma tumor suppressor gene. The retinoblastoma tumor suppressor gene product, known as Rb or pRb, regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1/S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, 2, 3)-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826. Hypophosphorylated Rb represses the transcription of genes controlling cell cycle through direct protein-protein interactions, by binding and inactivating the promoters of transcription factors, and through recruitment of histone deacetylase, which deacetylates promoter regions and enhances nucleosome formation, thereby masking transcription enhancing cis elements.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RB1 Antibody (NB100-91947)(1)

Publication using NB100-91947

• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (Human)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

FAQs for RB1 Antibody (NB100-91947). (Showing 1 - 1 of 1 FAQ).

1. My research group wants to order a Retinoblastoma 1 Antibody for IHC-Paraffin. We already bought 2 antibodies from other companies and they didn't work properly. There was always a cytoplasmatic staining and no nuclear staining. I found two antibodies on your website: Retinoblastoma 1 Antibody (7E4B8) (NBP1-51527) (0.1 ml) and Retinoblastoma 1 Antibody (IF8) (NB100-65771) (0.1 ml). Can you suggest which antibody we should buy? We are using a VENTANA BenchMark ULTRA stainer, FYI. We stain human material.
   • Retinoblastoma 1 Antibody (1F8-Rb1) (NB120-3077) (0.5 ml) is one of our very high quality product that has been validated for several applications in human samples. Alternatively you can choose Retinoblastoma 1 Antibody (NB100-91947) (0.1 mg) or Retinoblastoma 1 Antibody (7E4B8) (NBP1-51527) (0.1 ml) as they both also have nice validation images for the IHC-P applications.

Bioinformatics

• Gene Symbol: RB1
• Entrez:
  • 5925(Human)
  • 19645(Mouse)
  • 24708(Rat)