RB1 Antibody (SY63-03)

Western Blot: RB1 Antibody (SY63-03) [NBP2-67453] - Analysis of RB1 on mouse lung tissue lysates with Rabbit anti-Rb antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 106 kDa Observed band size: 106 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: RB1 Antibody (SY63-03) [NBP2-67453] - ICC staining of RB1 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary ant

Immunohistochemistry-Paraffin: RB1 Antibody (SY63-03) [NBP2-67453] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-RB1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: RB1 Antibody (SY63-03) [NBP2-67453] - Western blot analysis of RB1 on mouse lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat An

Immunohistochemistry-Paraffin: RB1 Antibody (SY63-03) [NBP2-67453] - Analysis of paraffin-embedded human spleen tissue using anti-Rb antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RB1 Antibody (SY63-03) [NBP2-67453] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Rb antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RB1 Antibody (SY63-03) [NBP2-67453] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-Rb antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: RB1 Antibody (SY63-03) [NBP2-67453] - Analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Rb antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: RB1 Antibody (SY63-03) [NBP2-67453] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-RB1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

RB1 Antibody (SY63-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within mouse RB1 aa 682-921 / 921. (SwissProt: P06400 Human; SwissProt: P13405 Mouse)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:2000
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RB1 Antibody (SY63-03)

• OSRC
• osteosarcoma
• p105-Rb
• pp110
• pRb
• RB
• RB1
• retinoblastoma 1
• retinoblastoma suspectibility protein
• retinoblastoma-associated protein

Background

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of ts function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RB1