RB1 Antibody (607121) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 607121
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

RB1 Antibody (607121) [Alexa Fluor® 350] Summary

• Immunogen: E.coli-derived recombinant human RB1
  Lys240-Asn406
  Accession # P06400
• Specificity: Detects human RB1 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RB1 Antibody (607121) [Alexa Fluor® 350]

• OSRC
• osteosarcoma
• p105-Rb
• pp110
• pRb
• RB
• RB1
• retinoblastoma 1
• retinoblastoma suspectibility protein
• retinoblastoma-associated protein

Background

Retinoblastoma 1 protein (RB-1; also retinoblastoma-associated protein, pp110, and p105-Rb) is a 110 kDa tumor suppressor gene and member of the retinoblastoma protein family.  Human RB-1 is 928 amino acids in length.  The protein contains a Pocket domain (aa 373-771), which is comprised of two other domains, domain A (aa 373-573) and domain B (aa 640-771), and a “spacer” (aa 580-639).  The Pocket domain binds to threonine-phosphorylated domain C (aa 771-928), which thereby prevents interaction with heterodimeric E2F/DP transcription factor complexes.  Human RB-1 is 90% aa identical to mouse RB-1.  RB-1 is expressed in the retina.  The underphosphorylated, active form of RB-1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest.  Defects in RB-1 lead to the childhood cancer retinoblastoma.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.