RB1 Antibody (1F8 (Rb1))

Images

 
Western Blot: RB1 Antibody (1F8-Rb1) [NB120-3077] - Analysis of 25 ug of K562 (Lane 1), B-3 (Lane 2) and rat eye (Lane 3) cell lysates.
Immunocytochemistry/ Immunofluorescence: RB1 Antibody (1F8-Rb1) [NB120-3077] - Analysis of Retinoblastoma (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary ...read more
Immunohistochemistry-Paraffin: RB1 Antibody (1F8-Rb1) [NB120-3077] - Human breast carcinoma stained with Retinoblastoma antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.
Flow Cytometry: RB1 Antibody (1F8-Rb1) [NB120-3077] - Analysis of RB1 in NIH/3T3 cells compared to an isotype control (blue).
Western Blot: RB1 Antibody (1F8-Rb1) [NB120-3077] - Analysis of Human Cells-17.
Flow Cytometry: RB1 Antibody (1F8-Rb1) [NB120-3077] - Analysis of Retinoblastoma in Daudi cells compared to an isotype control (blue).
Flow Cytometry: RB1 Antibody (1F8-Rb1) [NB120-3077] - Analysis of RB1 in Raji cells compared to an isotype control (blue).
Immunoprecipitation: RB1 Antibody (1F8-Rb1) [NB120-3077] - Native IP analysis.
Immunoprecipitation: RB1 Antibody (1F8-Rb1) [NB120-3077] - Denatured IP analysis.
Immunoprecipitation: RB1 Antibody (1F8-Rb1) [NB120-3077] - Analysis of Native Human LS174T Cells.
Immunoprecipitation: RB1 Antibody (1F8-Rb1) [NB120-3077] - Analysis of denatured Human HT29 Cells.

Product Details

Summary
Product Discontinued
View other related RB1 Primary Antibodies

Order Details


    • Catalog Number
      NB120-3077
    • Availability
      Product Discontinued

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RB1 Antibody (1F8 (Rb1)) Summary

Immunogen
Recombinant human Rb protein
Localization
Nuclear
Marker
Nuclear Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:200
  • Immunohistochemistry 1 - 2 ug/ml
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • Immunoprecipitation 2 ug/ml
Theoretical MW
106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Bladder Whole Tissue Lysate (Adult Whole Tumor)
RB1 Knockout HeLa Cell Lysate
Publications
Read Publications using
NB120-3077 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 15885897). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10mM PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for RB1 Antibody (1F8 (Rb1))

  • OSRC
  • osteosarcoma
  • p105-Rb
  • pp110
  • pRb
  • RB
  • RB1
  • retinoblastoma 1
  • retinoblastoma suspectibility protein
  • retinoblastoma-associated protein

Background

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of ts function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RB1 Antibody (NB120-3077)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(2)
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Filter By Species
Human
(2)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RB1 Antibody (NB120-3077). (Showing 1 - 3 of 3 FAQs).

  1. My research group wants to order a Retinoblastoma 1 Antibody for IHC-Paraffin. We already bought 2 antibodies from other companies and they didn't work properly. There was always a cytoplasmatic staining and no nuclear staining. I found two antibodies on your website: Retinoblastoma 1 Antibody (7E4B8) (NBP1-51527) (0.1 ml) and Retinoblastoma 1 Antibody (IF8) (NB100-65771) (0.1 ml). Can you suggest which antibody we should buy? We are using a VENTANA BenchMark ULTRA stainer, FYI. We stain human material.
    • Retinoblastoma 1 Antibody (1F8-Rb1) (NB120-3077) (0.5 ml) is one of our very high quality product that has been validated for several applications in human samples. Alternatively you can choose Retinoblastoma 1 Antibody (NB100-91947) (0.1 mg) or Retinoblastoma 1 Antibody (7E4B8) (NBP1-51527) (0.1 ml) as they both also have nice validation images for the IHC-P applications.
  2. Our customer would like to know if NB120-3077 would recognize both phosphorylated and non-phosphorylated Retinoblastoma 1, or only non-phosphorylated Retinoblastoma 1?
    • Retinoblastoma 1 Antibody (1F8-Rb1), catalog number NB120-3077, will recognize both phosphorylated as well as non-phosphorylated Retinoblastoma 1. Technically speaking, only antibodies raised to specific phospho-site recognize JUST phosphorylated form of a given target. Non-phospho specific antibodies detect total target (phospho + non-phospho).
  3. Hi could you by chance tell me what region was used to raise this antibody: Retinoblastoma 1 Antibody (1F8-Rb1) or if it was raised against full length.
    • The immunogen for this product was the full length human retinoblastoma recombinant protein.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RB1
Entrez
Uniprot