RAX Antibody (4F4) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: RAX Antibody (4F4) [H00030062-M02] - Analysis of monoclonal antibody to RAX on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: RAX Antibody (4F4) [H00030062-M02] - Analysis of monoclonal antibody to RAX on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 3 ug/ml.

ELISA: RAX Antibody (4F4) [H00030062-M02] - Detection limit for recombinant GST tagged RAX is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RAX Antibody (4F4) - Azide and BSA Free Summary

• Immunogen: RAX (NP_038463, 104 a.a. ~ 206 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KEPWEARPSPGLPVGPATGEAKLSEEEQPKKKHRRNRTTFTTYQLHELERAFEKSHYPDVYSREELAGKVNLPEVRVQVWFQNRRAKWRRQEKLEVSSMKLQD
• Specificity: RAX (4F4)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAX
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for western blot. It has also been used for ELISA and immunohistochemistry (paraffin).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RAX Antibody (4F4) - Azide and BSA Free

• MCOP3
• retina and anterior neural fold homeobox
• retinal homeobox protein Rx
• RXRetina and anterior neural fold homeobox protein

Background

The RAX gene encodes a homeobox-containing transcription factor that functions in eye development. The gene is expressed early in the eye primordia, and is required for retinal cell fate determination and also regulates stem cell proliferation. Mutations in

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAX Antibody (H00030062-M02)(1)

Publication using H00030062-M02

• Shrestha R, Wen YT, Ding DC, Tsai RK et al. Aberrant hiPSCs-Derived from Human Keratinocytes Differentiates into 3D Retinal Organoids that Acquire Mature Photoreceptors. Cells. 2019-03-09 [PMID: 30634512] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: RAX
• Entrez:
  • 30062(Human)
• OMIM:
  • 601881(Human)
• Uniprot:
  • NP_038463(Human)