RASSF3 Antibody

Western Blot: RASSF3 Antibody [NBP1-20967] - Analysis of RASSF3 in Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using NBP1-20967 at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RASSF3 Antibody Summary

• Immunogen: Peptide with sequence CKPPQTSPNSGK corresponding to C-Terminus according to NP_835463.1.
• Epitope: internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RASSF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in human heart lysates (calculated MW of 27.6 kDa band according to NP_835463.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RASSF3 Antibody

• MGC119194
• MGC119195
• MGC119197
• Ras association (RalGDS/AF-6) domain family 3
• Ras association (RalGDS/AF-6) domain family member 3
• ras association domain-containing protein 3
• RASSF5

Background

The RAS oncogene (MIM 190020) is mutated in nearly one-third of all human cancers. Members of the RAS superfamily are plasma membrane GTP-binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including RASSF3, share a RAS association (RA) domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RASSF3 Antibody (NBP1-20967)(1)

Publication using NBP1-20967

• Tommasi S, Dammann R, Jin SG et al. RASSF3 and NORE1: identification and cloning of two human homologues of the putative tumor suppressor gene RASSF1. Oncogene 2002-04-18 [PMID: 11965544]

Bioinformatics

• Gene Symbol: RASSF3
• Entrez:
  • 283349(Human)
• Uniprot:
  • NP_835463.1(Human)