RASSF1 Antibody

Product Discontinued

RASSF1 Antibody Summary

• Immunogen: Peptide with sequence C-QAETEQKIKDYNGQ corresponding to internal region according to Uniprot Mouse NP_062687.1.
• Epitope: C-QAETEQKIKDYNGQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RASSF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Preliminary experiments in mouse and rat kidney and liver lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for RASSF1 Antibody

• 123F2
• NORE2A
• pancreas-specific ras association domain family 1 protein
• Ras association (RalGDS/AF-6) domain family member 1
• ras association domain-containing protein 1
• RASSF1A
• RDA32cardiac-specific ras association domain family 1 protein
• REH3P21
• tumor suppressor protein RDA32
• WUGSC:H_LUCA12.5

Background

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability. Agathanggelou A. et al

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RASSF1 Antibody (NBP1-06068)(1)

Publication using NBP1-06068

• Yan PS, Venkataramu C, Ibrahim A et al. Mapping geographic zones of cancer risk with epigenetic biomarkers in normal breast tissue. Clin Cancer Res 2006-11-15 [PMID: 17121881]

Bioinformatics

• Gene Symbol: RASSF1
• Entrez:
  • 56289(Mouse)
  • 363140(Rat)
• Uniprot:
  • NP_062687.1(Mouse)