RASSF1 Antibody (3F3)

Images

 
Western Blot: RASSF1 Antibody (3F3) [NBP1-04342] - The extracts of HEK293 (1), A549 (2) and HeLa (3) cells were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins ...read more
Immunocytochemistry/ Immunofluorescence: RASSF1 Antibody (3F3) [NBP1-04342] - A431 cells were labeled with anti-RASSF1A (clone 3F3) mAb, and detection was using a biotinylated secondary antibody and Texas-red conj ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
3F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

RASSF1 Antibody (3F3) Summary

Immunogen
Recombinant human RASSF1A (1-340 aa), purified from E. coli (NP_009113).
Specificity
Anti-human RASSF1A mAb, clone 3F3, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human RASSF1A protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RASSF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
The antibody has been tested by ELISA,Western blot and immunocytochemistry analysis to assure specificity and reactivity.
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
A-431 Lysate (NBL1-26466)
HeLa Lysate (NB820-59462)

Reactivity Notes

This antibody is reactive against Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for RASSF1 Antibody (3F3)

  • 123F2
  • NORE2A
  • pancreas-specific ras association domain family 1 protein
  • Ras association (RalGDS/AF-6) domain family member 1
  • ras association domain-containing protein 1
  • RASSF1A
  • RDA32cardiac-specific ras association domain family 1 protein
  • REH3P21
  • tumor suppressor protein RDA32
  • WUGSC:H_LUCA12.5

Background

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability. Agathanggelou A. et al

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RASSF1 Antibody (NBP1-04342) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-04342AF405
Alexa Fluor 488 Labeled NBP1-04342AF488
Alexa Fluor 647 Labeled NBP1-04342AF647
Alexa Fluor 700 Labeled NBP1-04342AF700
DyLight 350 Labeled NBP1-04342UV
DyLight 405 Labeled NBP1-04342V
DyLight 488 Labeled NBP1-04342G
DyLight 680 Labeled NBP1-04342FR
DyLight 755 Labeled NBP1-04342IR

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Bioinformatics Tool for RASSF1 Antibody (NBP1-04342)

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Blogs on RASSF1.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

Role of RASSF1A in Death Receptor-Dependent Apoptosis
Death-receptor apoptosis, or cell death, is essential for cellular growth regulation; its disruption is expressed in a variety of cancers. We at Novus Biologicals are one of the leading antibody suppliersfor apoptosis and cancer research groups, and t...  Read full blog post.

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Bioinformatics

Gene Symbol RASSF1
Entrez
Uniprot