RAP74 Antibody

Western Blot: RAP74 Antibody [NBP2-94406] - Analysis of extracts of various cell lines, using RAP74 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry/Immunofluorescence: RAP74 Antibody [NBP2-94406] - Immunofluorescence analysis of A549 cells using RAP74 antibody (NBP2-94406). Blue: DAPI for nuclear staining.

Product Discontinued

RAP74 Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human GTF2F2 (NP_004119.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF2F2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:2000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RAP74 Antibody

• BTF4
• General transcription factor IIF 74 kDa subunit
• general transcription factor IIF subunit 1
• general transcription factor IIF, polypeptide 1, 74kDa
• TF2F1
• TFIIF
• TFIIF-alpha
• Transcription initiation factor IIF subunit alpha
• transcription initiation factor RAP74

Background

Enables several functions, including RNA polymerase II general transcription initiation factor activity; phosphatase activator activity; and promoter-specific chromatin binding activity. Involved in several processes, including positive regulation of transcription by RNA polymerase II; response to virus; and transcription initiation from RNA polymerase II promoter. Located in cell junction and nucleoplasm. Part of transcription factor TFIID complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2F2