RAP30 Antibody

Images

 
Western Blot: RAP30 Antibody [NBP3-17973] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-459
Immunocytochemistry/ Immunofluorescence: RAP30 Antibody [NBP3-17973] - Staining of human cell line U-2 OS shows positivity in nucleus and cytoskeleton (microtubules).
Immunohistochemistry: RAP30 Antibody [NBP3-17973] - Staining of human rectum shows strong nuclear positivity in glandular cells.
Immunocytochemistry/ Immunofluorescence: RAP30 Antibody [NBP3-17973] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & microtubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RAP30 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVVYLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEEK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GTF2F2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RAP30 Antibody

  • ATP-dependent helicase GTF2F2
  • BTF4
  • General transcription factor IIF 30 kDa subunit
  • general transcription factor IIF subunit 2
  • general transcription factor IIF, polypeptide 2, 30kDa
  • TF2F2
  • TFIIF
  • TFIIF-beta
  • Transcription initiation factor IIF subunit beta
  • Transcription initiation factor RAP30

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RAP30 Antibody (NBP3-17973) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GTF2F2