Recombinant Human RAP1A His Protein

SDS-Page: Recombinant Human RAP1A Protein [NBP1-45263] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human RAP1A His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-181 of Human RAP1A
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: RAP1A
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 22.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 40% glycerol, 5mM DTT, 200 mM NaCl
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human RAP1A His Protein

• C21KG
• G-22K
• GTP-binding protein smg p21A
• KREV-1
• KREV1Ras-related protein Krev-1
• RAP1
• Rap1A
• RAP1A, member of RAS oncogene family
• RAS-related protein RAP1A
• ras-related protein Rap-1A
• SMGP21

Background

Ras-related protein Rap-1A (RAP1A) belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. This Protein Induces morphological reversion of a cell line transformed by a Ras oncogene and Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Recombinant human RAP1A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RAP1A