Human RAP1A ELISA Kit (Colorimetric)

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Standard Curve Reference.
Samples were spiked with high concentrations of Human RAP1A, and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.1 ng/mL

Order Details

Human RAP1A ELISA Kit (Colorimetric) Summary

Description
Assay Length: 3 h 30 min
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.1 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10
Intra-Assay
CV% < 10
Spike Recovery
80 - 120%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
RAP1A

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100x)
  4. Concentrated HRP Conjugate (100x)
  5. Concentrated Wash Buffer (25x)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate (Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Human RAP1A ELISA Kit (Colorimetric)

  • C21KG
  • G-22K
  • GTP-binding protein smg p21A
  • KREV-1
  • KREV1Ras-related protein Krev-1
  • RAP1
  • Rap1A
  • RAP1A, member of RAS oncogene family
  • RAS-related protein RAP1A
  • ras-related protein Rap-1A
  • SMGP21

Background

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for RAP1A ELISA Kit (NBP3-49899). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol RAP1A