Recombinant Human Ran Protein

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SDS-Page: Recombinant Human Ran Protein [NBP1-45255] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Ran Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-216 of Human Ran

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
RAN
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
26.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Ran Protein

  • Androgen receptor-associated protein 24
  • ARA24Gsp1
  • GTPase Ran
  • GTP-binding nuclear protein Ran
  • guanosine triphosphatase Ran
  • member RAS oncogene family
  • OK/SW-cl.81
  • RAN, member RAS oncogene family
  • RanGTPase
  • Ras-like protein TC4
  • Ras-related nuclear protein
  • TC4

Background

RAN, member RAS oncogene family, also known as RAN, is a small GTPase of the Ras superfamily that controls nucleocytoplasmic transport. It is involved in the directionality of the process that is regulated by GTP hydrolysis. Ran shuttles between the nucleus and the cytoplasm. Unlike many other regulatory GTPases, Ran is not posttranslationally modified so that it can be targeted to the subcellular compartment where it acts. Rather, RanGTP has been thought to be localized by the differential localization of Ran regulators. Recombinant human RAN, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for Ran Recombinant Protein (NBP1-45255) (0)

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FAQs for Ran Recombinant Protein (NBP1-45255). (Showing 1 - 2 of 2 FAQ).

  1. If Ran is not in its GTP form, how to get Ran-GTP? (buffer, reaction time and temperature, GTP kind, etc)
    • We do not anticipate that this protein is active, and it is not recommend for functional assay. NBP1-45255 has been validated in ELISA (for standard curve) and PAGE (protein control) only. If wanting to perform functional assay, the mammalian cell expressed Ran protein will be more suitable. Unfortunately I do not have such information, but can only provide you the general references for you to check: Woo IS., et al. (2008) Apoptosis. 13(10):1223-31 and Ren M., et al. (1994) Mol Cell biol. 14(6):4216-24.
  2. Is this protein good for RNA nuclear export assay?
    • Since we do not provide any active Ran, we do not have recommended protocol for RNA nuclear export assay.

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Blogs on Ran.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

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Bioinformatics

Gene Symbol RAN