RalB Antibody (399417)

Western blot shows lysates of U937 human histiocytic lymphoma cell line and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human RalB Monoclonal Antibody (Catalog # MAB3920) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human RalA and RalB (10 ng/lane) were included. A specific band for RalB was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 399417
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

RalB Antibody (399417) Summary

• Immunogen: E. coli-derived recombinant human RalB
  Met1-Leu206
  Accession # P11234
• Specificity: Detects human, mouse, and rat RalB in Western blots. In Western blots, no cross-reactivity with recombinant human RalA is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: RALB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RalB Antibody (399417)

• GTP binding protein
• RalB
• RAS-like protein B
• ras-related protein Ral-B
• v-ral simian leukemia viral oncogene homolog B (ras related; GTP bindingprotein)

Background

The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RalB Antibody (MAB3920)(2)

Publications using MAB3920

• Andrea Ommer, Gianluca Figlia, Jorge A. Pereira, Anna Lena Datwyler, Joanne Gerber, Jonathan DeGeer et al. Ral GTPases in Schwann cells promote radial axonal sorting in the peripheral nervous system Journal of Cell Biology 2019-07-01 [PMID: 31201267]
• Smith , Steven C, Baras , Alexande, Owens , Charles, Dancik , Garrett, Theodorescu , Dan Transcriptional signatures of Ral GTPase are associated with aggressive clinicopathologic characteristics in human cancer. Cancer Res, 2012-05-14;72(14):3480-91. 2012-05-14 [PMID: 22586063] (IHC-P, Human)

Bioinformatics

• Gene Symbol: RALB
• Uniprot:
  • P11234()