RAGEF2 Antibody

Product Discontinued

RAGEF2 Antibody Summary

• Immunogen: Synthetic peptide, TKNLSEKRSAKII representing the C-terminus of the human protein (residues 1192-12) according to NP_057424.
• Localization: Upon binding to M-Ras, it translocates to the plasma membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RAPGEF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA detection limit 1:32000
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments showed a strong band at approx 60-70kDa accompanied by weaker band at approx 110-120kDa in Human Brain at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 138Kda according to NP_057424. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for RAGEF2 Antibody

• DKFZp667N084
• DKFZp686I15116
• KIA001LB
• PDZ domain-containing guanine nucleotide exchange factor 2
• PDZ domain-containing guanine nucleotide exchange factor I
• PDZ-GEF2RA-GEF-2PDZGEF2PDZ domain containing guanine nucleotide exchange factor (GEF) 2
• RAGEF2
• Rap guanine nucleotide exchange factor (GEF) 6
• rap guanine nucleotide exchange factor 6

Background

Low similarity to human CAMP-GEFII; may be a membrane signalling protein; contains a PDZ, a cyclic nucleotide-binding, and a RasGEF domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAPGEF6
• Entrez:
  • 51735(Human)
• Uniprot:
  • NP_057424.(Human)