Raf-1 [p Ser233] Antibody (Roy/28/1)

Images

 

Product Details

Summary
Product Discontinued
View other related Raf-1 Primary Antibodies

Order Details


    • Catalog Number
      NB100-66306
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Raf-1 [p Ser233] Antibody (Roy/28/1) Summary

Immunogen
Made to Human RAF-1
Modification
p Ser233
Specificity
NB100-66306 recognizes Raf-1 phosphorylated at the serine residue at position 233. The antibody binds to Raf-1 that has been activated by cAMP activity at the newly identified serine 233 position, but not to resting, Raf-1. The antibody does not bind to Raf peptides phosphorylated at other sites.
Isotype
IgG
Clonality
Monoclonal
Host
Rat
Gene
RAF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:1000
Application Notes
For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other caseinbased blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Raf-1 [p Ser233] Antibody (Roy/28/1)

  • cRaf
  • c-Raf
  • c-Raf-1
  • CRAFraf proto-oncogene serine/threonine protein kinase
  • EC 2.7.11
  • EC 2.7.11.1
  • NS5
  • Proto-oncogene c-RAF
  • RAF proto-oncogene serine/threonine-protein kinase
  • RAF
  • Raf1
  • Raf-1
  • Raf-1Oncogene RAF1
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • v-Raf-1

Background

The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-47833
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-13304
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
AF1347
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP2-43792
Species: Hu
Applications: WB
H00003845-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
NBP2-45745
Species: Hu
Applications: WB, IHC, IF
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NBP2-46403
Species: Hu
Applications: IHC
NBP1-89942
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for Raf-1 Antibody (NB100-66306) (0)

There are no publications for Raf-1 Antibody (NB100-66306).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Raf-1 Antibody (NB100-66306) (0)

There are no reviews for Raf-1 Antibody (NB100-66306). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Raf-1 Antibody (NB100-66306) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Raf-1 Products

Bioinformatics Tool for Raf-1 Antibody (NB100-66306)

Discover related pathways, diseases and genes to Raf-1 Antibody (NB100-66306). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Raf-1 Antibody (NB100-66306)

Discover more about diseases related to Raf-1 Antibody (NB100-66306).
 

Pathways for Raf-1 Antibody (NB100-66306)

View related products by pathway.

PTMs for Raf-1 Antibody (NB100-66306)

Learn more about PTMs related to Raf-1 Antibody (NB100-66306).
 

Research Areas for Raf-1 Antibody (NB100-66306)

Find related products by research area.

Blogs on Raf-1

There are no specific blogs for Raf-1, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Raf-1 [p Ser233] Antibody (Roy/28/1) and receive a gift card or discount.

Bioinformatics

Gene Symbol RAF1
Entrez
Uniprot