Raf-1 Antibody (JJ086-06)

Western Blot: Raf-1 Antibody (JJ086-06) [NBP2-67847] - Analysis of Raf1 on HepG2 cells lysates using anti-Raf1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Raf-1 Antibody (JJ086-06) [NBP2-67847] - ICC staining Raf-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Raf-1 Antibody (JJ086-06) [NBP2-67847] - ICC staining Raf-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Product Discontinued

Raf-1 Antibody (JJ086-06) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human Raf-1 aa 1-50/648. (SwissProt: P04049 Human)
• Localization: Cytoplasm, Cell membrane, Mitochondrion, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RAF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Raf-1 Antibody (JJ086-06)

• cRaf
• c-Raf
• c-Raf-1
• CRAFraf proto-oncogene serine/threonine protein kinase
• EC 2.7.11
• EC 2.7.11.1
• NS5
• Proto-oncogene c-RAF
• RAF proto-oncogene serine/threonine-protein kinase
• RAF
• Raf1
• Raf-1
• Raf-1Oncogene RAF1
• v-raf-1 murine leukemia viral oncogene homolog 1
• v-Raf-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAF1