RAE1 Antibody - BSA Free

Western Blot: RAE1 Antibody [NBP1-31027] - Sample(30 ug of whole cell lysate)A:HeLa S3 12% SDS PAGE, antibody diluted at 1:1500.

Immunocytochemistry/Immunofluorescence: RAE1 Antibody [NBP1-31027] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAE1 protein stained by RAE1 antibody [C3], C-term diluted at 1:500.Blue: Hoechst 33342 staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Pm, Xp, Ze
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RAE1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RAE1 Antibody - BSA Free (NBP1-31027) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RAE1. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Zebrafish (100%), Chicken (100%), Primate (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAE1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RAE1 Antibody - BSA Free

• dJ481F12.3
• dJ800J21.1
• FLJ30608
• homolog of yeast Rae1 (Bharathi) mRNA-associated protein of 41 kDa (Kraemer)
• MGC117333
• MGC126076
• MGC126077
• MIG14
• migration-inducing gene 14
• Mnrp41
• mRNA export factor
• mRNA export protein
• mRNA-associated protein mrnp 41
• mRNA-binding protein, 41-kD
• MRNP41
• RAE1 (RNA export 1, S.pombe) homolog
• Rae1 protein homolog
• RAE1 RNA export 1 homolog (S. pombe)

Background

Mutations in the Schizosaccharomyces pombe Rae1 and Saccharomyces cerevisiae Gle2 genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. The protein encoded by this gene is a homolog of yeast Rae1. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RAE1