Rae-1 Antibody (186107) [Unconjugated] - Pan Specific

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific Monoclonal Antibody (Catalog # MAB17582, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 186107
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Rae-1 Antibody (186107) [Unconjugated] - Pan Specific Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
  Leu29-Ser227
  Accession # Q9JI58
• Specificity: Detects mouse Rae‑1. It recognizes Rae-1 alpha , beta , δ, gamma and epsilon .
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Raet1a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rae-1 Antibody (186107) [Unconjugated] - Pan Specific

• dJ481F12.3;dJ800J21.1;Gle2;MIG14;Mnrp41;mRNA export factor RAE1;MRNP41;RAE1
• Rae1
• Rae-1

Background

Rae-1 alpha , beta , gamma , δ and epsilon comprise a family of closely related (88-95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rae-1 Antibody (MAB17582)(26)

Publications using MAB17582

• R Gund, AM Christiano Impaired autophagy promotes hair loss in the C3H/HeJ mouse model of alopecia areata Autophagy, 2022-06-02;0(0):1-10. 2022-06-02 [PMID: 35652954] (ICC, Mouse)
• JH Park, HJ Kim, CW Kim, HC Kim, Y Jung, HS Lee, Y Lee, YS Ju, JE Oh, SH Park, JH Lee, SK Lee, HK Lee Tumor hypoxia represses &gamma&delta T cell-mediated antitumor immunity against brain tumors Nature Immunology, 2021-02-11;0(0):. 2021-02-11 [PMID: 33574616] (Flow Cytometry, Mouse)
• D Prinz, K Klein, J List, VM Knab, I Menzl, N Leidenfros, G Heller, B Poli?, EM Putz, A Witalisz-S, V Sexl, D Gotthardt Loss of NKG2D in murine NK cells leads to increased perforin production upon long-term stimulation with IL-2 Eur. J. Immunol., 2020-02-20;0(0):. 2020-02-20 [PMID: 32052406] (Flow Cytometry, Mouse)
• G D'Alessand, F Antonangel, F Marrocco, A Porzia, C Lauro, A Santoni, C Limatola Gut microbiota alterations affect glioma growth and innate immune cells involved in tumor immunosurveillance in mice Eur. J. Immunol., 2020-03-01;0(0):. 2020-03-01 [PMID: 32034922] (Flow Cytometry, Mouse)
• K Dai, Y Huang, Z Chen, X Sun, L Yang, Y Jiang Kbtbd2 inhibits the cytotoxic activity of immortalized NK cells through down-regulating mTOR signaling in a mouse hepatocellular carcinoma model Eur. J. Immunol., 2018-01-29;0(0):. 2018-01-29 [PMID: 29331106] (Flow Cytometry, Mouse)
• Morvan M, Champsaur M, Reizis B, Lanier L Chronic In Vivo Interaction of Dendritic Cells Expressing the Ligand Rae-1epsilon with NK Cells Impacts NKG2D Expression and Function. Immunohorizons, 2017-05-01;1(3):10-19. 2017-05-01 [PMID: 28815225] (Flow Cytometry, Mouse)
• A Herpesviral induction of RAE-1 NKG2D ligand expression occurs through release of HDAC mediated repression Elife, 2016-11-22;5(0):. 2016-11-22 [PMID: 27874833] (Flow Cytometry, Mouse)
• NKG2D-NKG2D Ligand Interaction Inhibits the Outgrowth of Naturally Arising Low-Grade B Cell Lymphoma In Vivo J Immunol, 2016-05-02;196(11):4805-13. 2016-05-02 [PMID: 27183590] (Flow Cytometry, Mouse)
• Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud V, Anjuere F NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine. PLoS ONE, 2015-12-02;10(12):e0143224. 2015-12-02 [PMID: 26630176] (Flow Cytometry, Mouse)
• Dupuis-Maurin V, Brinza L, Baguet J, Plantamura E, Schicklin S, Chambion S, Macari C, Tomkowiak M, Deniaud E, Leverrier Y, Marvel J, Michallet M Overexpression of the transcription factor Sp1 activates the OAS-RNAse L-RIG-I pathway. PLoS ONE, 2015-03-04;10(3):e0118551. 2015-03-04 [PMID: 25738304] (Flow Cytometry, Mouse)
• Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. J Invest Dermatol, 2015-01-29;135(5):1311-9. 2015-01-29 [PMID: 25634359] (Flow Cytometry, Western Blot, Mouse)
• Weinger J, Plaisted W, Maciejewski S, Lanier L, Walsh C, Lane T Activating receptor NKG2D targets RAE-1-expressing allogeneic neural precursor cells in a viral model of multiple sclerosis. Stem Cells, 2014-10-01;32(10):2690-701. 2014-10-01 [PMID: 24898518] (Flow Cytometry, Mouse)
• Tu M, Mahmoud A, Wight A, Mottashed A, Belanger S, Rahim M, Abou-Samra E, Makrigiannis A Ly49 family receptors are required for cancer immunosurveillance mediated by natural killer cells. Cancer Res, 2014-05-06;74(14):3684-94. 2014-05-06 [PMID: 24802191] (Flow Cytometry, Mouse)
• Crosby E, Goldschmidt M, Wherry E, Scott P Engagement of NKG2D on bystander memory CD8 T cells promotes increased immunopathology following Leishmania major infection. PLoS Pathog, 2014-02-27;10(2):e1003970. 2014-02-27 [PMID: 24586170] (Flow Cytometry, Mouse)
• Paolino , Magdalen, Choidas , Axel, Wallner , Stephani, Pranjic , Blanka, Uribesalgo , Iris, Loeser , Stefanie, Jamieson , Amanda M, Langdon , Wallace, Ikeda , Fumiyo, Fededa , Juan Pab, Cronin , Shane J, Nitsch , Roberto, Schultz-Fademrecht , Carsten, Eickhoff , Jan, Menninger , Sascha, Unger , Anke, Torka , Robert, Gruber , Thomas, Hinterleitner , Reinhard, Baier , Gottfrie, Wolf , Dominik, Ullrich , Axel, Klebl , Bert M, Penninger , Josef M The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells. Nature, 2014-02-19;507(7493):508-12. 2014-02-19 [PMID: 24553136] (Flow Cytometry, Mouse)
• Rui Wang, Kannan Natarajan, Maria Jamela R. Revilleza, Lisa F. Boyd, Li Zhi, Huaying Zhao et al. Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1 gamma reveals a paradigm for MHC/MHC interaction in immune evasion Proceedings of the National Academy of Sciences 12/18/2012 [PMID: 23169621]
• Rui Wang, Kannan Natarajan, Maria Jamela R. Revilleza, Lisa F. Boyd, Li Zhi, Huaying Zhao et al. Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1 gamma reveals a paradigm for MHC/MHC interaction in immune evasion Proceedings of the National Academy of Sciences 2012-12-18 [PMID: 23169621] (Surface Plasmon Resonance, Mouse)
• Jung , Heiyoun, Hsiung , Benjamin, Pestal , Kathleen, Procyk , Emily, Raulet , David H RAE-1 ligands for the NKG2D receptor are regulated by E2F transcription factors, which control cell cycle entry. J Exp Med, 2012-11-19;209(13):2409-22. 2012-11-19 [PMID: 23166357] (Flow Cytometry, Mouse)
• Borchers MT, Wesselkamper SC, Curull V, Ramirez-Sarmiento A, Sanchez-Font A, Garcia-Aymerich J, Coronell C, Lloreta J, Agusti AG, Gea J, Howington JA, Reed MF, Starnes SL, Harris NL, Vitucci M, Eppert BL, Motz GT, Fogel K, McGraw DW, Tichelaar JW, Orozco-Levi M Sustained CTL activation by murine pulmonary epithelial cells promotes the development of COPD-like disease. J. Clin. Invest., 2009-02-09;119(3):636-49. 2009-02-09 [PMID: 19197141] (Flow Cytometry, Mouse)
• Timothy J. Nice, Laurent Coscoy, David H. Raulet Posttranslational regulation of the NKG2D ligand Mult1 in response to cell stress Journal of Experimental Medicine 2/16/2009 [PMID: 19171762]
• Timothy J. Nice, Laurent Coscoy, David H. Raulet Posttranslational regulation of the NKG2D ligand Mult1 in response to cell stress Journal of Experimental Medicine 2009-02-16 [PMID: 19171762] (Flow Cytometry, Mouse)
• Unni AM, Bondar T, Medzhitov R Intrinsic sensor of oncogenic transformation induces a signal for innate immunosurveillance. Proc. Natl. Acad. Sci. U.S.A., 2008-01-25;105(5):1686-91. 2008-01-25 [PMID: 18223157] (Flow Cytometry, Mouse)
• Vilarinho S, Ogasawara K, Nishimura S, Lanier LL, Baron JL Blockade of NKG2D on NKT cells prevents hepatitis and the acute immune response to hepatitis B virus. Proc. Natl. Acad. Sci. U.S.A., 2007-11-08;104(46):18187-92. 2007-11-08 [PMID: 17991774] (Flow Cytometry, Mouse)
• Barber A, Zhang T, DeMars LR, Conejo-Garcia J, Roby KF, Sentman CL Chimeric NKG2D receptor-bearing T cells as immunotherapy for ovarian cancer. Cancer Res., 2007-05-15;67(10):5003-8. 2007-05-15 [PMID: 17510432] (Flow Cytometry, Mouse)
• Gourzi P, Leonova T, Papavasiliou FN A role for activation-induced cytidine deaminase in the host response against a transforming retrovirus. Immunity, 2006-06-01;24(6):779-86. 2006-06-01 [PMID: 16782033] (Flow Cytometry, Mouse)
• Terme M, Borg C, Guilhot F, Masurier C, Flament C, Wagner EF, Caillat-Zucman S, Bernheim A, Turhan AG, Caignard A, Zitvogel L BCR/ABL promotes dendritic cell-mediated natural killer cell activation. Cancer Res., 2005-07-15;65(14):6409-17. 2005-07-15 [PMID: 16024645] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Raet1a
• Uniprot:
  • Q9JI58()