Rae-1 Antibody (186107) [Phycoerythrin] - Pan Specific

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific PE-conjugated Monoclonal Antibody (Catalog # FAB17582P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Used 1uL for 15mins at 4°C on AT-3 cells transduced to over express murine Rae-1. Image from a verified customer review.

• Reactivity: Mu
• Applications: Flow
• Clone: 186107
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

Rae-1 Antibody (186107) [Phycoerythrin] - Pan Specific Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
  Leu29-Ser227
  Accession # Q9JI58
• Specificity: Detects mouse Rae‑1. It recognizes Rae-1 alpha , beta , δ, gamma and epsilon .
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Raet1a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rae-1 Antibody (186107) [Phycoerythrin] - Pan Specific

• dJ481F12.3;dJ800J21.1;Gle2;MIG14;Mnrp41;mRNA export factor RAE1;MRNP41;RAE1
• Rae1
• Rae-1

Background

Rae-1 alpha , beta , gamma , δ and epsilon comprise a family of closely related (88-95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rae-1 Antibody (FAB17582P)(13)

Publications using FAB17582P

• Chang, YH;Yamamoto, K;Fujino, T;Wang, TW;Sugimoto, E;Zhang, W;Yabushita, T;Suzaki, K;Pietsch, EC;Weir, BA;Crescenzo, R;Cowley, GS;Attar, R;Philippar, U;Wunderlich, M;Mizukawa, B;Zheng, Y;Enomoto, Y;Imai, Y;Kitamura, T;Goyama, S; SETDB1 suppresses NK cell-mediated immunosurveillance in acute myeloid leukemia with granulo-monocytic differentiation Cell reports 2024-07-30 [PMID: 39096901] (Flow Cytometry, Mouse)
• Wang, Z;Li, W;Jiang, Y;Tran, TB;Cordova, LE;Chung, J;Kim, M;Wondrak, G;Erdrich, J;Lu, J; Sphingomyelin-derived nanovesicles for the delivery of the IDO1 inhibitor epacadostat enhance metastatic and post-surgical melanoma immunotherapy Nature communications 2023-11-09 [PMID: 37945606] (Flow Cytometry, Mouse)
• José A. Casado, Antonio Valeri, Rebeca Sanchez-Domínguez, Paula Vela, Andrea López, Susana Navarro et al. Upregulation of NKG2D ligands impairs hematopoietic stem cell function in Fanconi anemia Journal of Clinical Investigation 8/1/2022 [PMID: 35671096]
• GK Griffin, J Wu, A Iracheta-V, JC Patti, J Hsu, T Davis, D Dele-Oni, PP Du, AG Halawi, JJ Ishizuka, SY Kim, S Klaeger, NH Knudsen, BC Miller, TH Nguyen, KE Olander, M Papanastas, S Rachimi, EJ Robitschek, EM Schneider, MD Yeary, MD Zimmer, JD Jaffe, SA Carr, JG Doench, WN Haining, KB Yates, RT Manguso, BE Bernstein Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity Nature, 2021-05-05;0(0):. 2021-05-05 [PMID: 33953401] (Flow Cytometry, Mouse)
• G Itakura, M Ozaki, N Nagoshi, S Kawabata, Y Nishiyama, K Sugai, T Iida, R Kashiwagi, T Ookubo, K Yastake, K Matsubayas, J Kohyama, A Iwanami, M Matsumoto, M Nakamura, H Okano Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells Sci Rep, 2017-10-11;7(1):12996. 2017-10-11 [PMID: 29021610] (Flow Cytometry, Mouse)
• Liu Q, Sanai N, Jin W, La Cava A, Van Kaer L, Shi F Neural stem cells sustain natural killer cells that dictate recovery from brain inflammation. Nat Neurosci, 2016-01-11;19(2):243-52. 2016-01-11 [PMID: 26752157] (Flow Cytometry, Mouse)
• Narumi K, Miyakawa R, Ueda R, Hashimoto H, Yamamoto Y, Yoshida T, Aoki K Proinflammatory Proteins S100A8/S100A9 Activate NK Cells via Interaction with RAGE. J Immunol, 2015-04-24;194(11):5539-48. 2015-04-24 [PMID: 25911757] (Flow Cytometry, Mouse)
• Zhan R, Han Q, Zhang C, Tian Z, Zhang J Toll-Like receptor 2 (TLR2) and TLR9 play opposing roles in host innate immunity against Salmonella enterica serovar Typhimurium infection. Infect Immun, 2015-02-09;83(4):1641-9. 2015-02-09 [PMID: 25667264] (Flow Cytometry, Mouse)
• Rui Wang, Kannan Natarajan, Maria Jamela R. Revilleza, Lisa F. Boyd, Li Zhi, Huaying Zhao et al. Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1 gamma reveals a paradigm for MHC/MHC interaction in immune evasion Proceedings of the National Academy of Sciences 2012-12-18 [PMID: 23169621] (Flow Cytometry, Mouse)
• Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. PLoS Pathog., 2012-01-12;8(1):e1002481. 2012-01-12 [PMID: 22253596] (Flow Cytometry, Mouse)
• Carayannopoulos LN, Barks JL, Yokoyama WM Murine trophoblast cells induce NK cell interferon-gamma production through KLRK1. Biol. Reprod., 2010-05-19;83(3):404-14. 2010-05-19 [PMID: 20484740] (Flow Cytometry, Mouse)
• Walsh KB, Lanier LL, Lane TE NKG2D receptor signaling enhances cytolytic activity by virus-specific CD8+ T cells: evidence for a protective role in virus-induced encephalitis. J. Virol., 2007-12-26;82(6):3031-44. 2007-12-26 [PMID: 18160433] (Flow Cytometry, Mouse)
• Ogasawara K, Hamerman JA, Ehrlich LR, Bour-Jordan H, Santamaria P, Bluestone JA, Lanier LL NKG2D blockade prevents autoimmune diabetes in NOD mice. Immunity, 2004-06-01;20(6):757-67. 2004-06-01 [PMID: 15189740] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Raet1a
• Uniprot:
  • Q9JI58()