Rae-1 Antibody (186107) [Allophycocyanin] - Pan Specific

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific APC-conjugated Monoclonal Antibody (Catalog # FAB17582A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 186107
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

Rae-1 Antibody (186107) [Allophycocyanin] - Pan Specific Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
  Leu29-Ser227
  Accession # Q9JI58
• Specificity: Detects mouse Rae‑1. It recognizes Rae-1 alpha , beta , δ, gamma and epsilon .
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Raet1a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rae-1 Antibody (186107) [Allophycocyanin] - Pan Specific

• dJ481F12.3;dJ800J21.1;Gle2;MIG14;Mnrp41;mRNA export factor RAE1;MRNP41;RAE1
• Rae1
• Rae-1

Background

Rae-1 alpha , beta , gamma , δ and epsilon comprise a family of closely related (88-95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rae-1 Antibody (FAB17582A)(8)

Publications using FAB17582A

• Y Kanda, A Takeuchi, M Ozawa, Y Kurosawa, T Kawamura, D Bogdanova, H Iioka, E Kondo, Y Kitazawa, H Ueta, K Matsuno, T Kinashi, T Katakai Visualizing the Rapid and Dynamic Elimination of Allogeneic T Cells in Secondary Lymphoid Organs J. Immunol., 2018-06-20;0(0):. 2018-06-20 [PMID: 29925676] (Flow Cytometry, Mouse)
• H Liu, AR Osterburg, J Flury, S Huang, FX McCormack, SA Cormier, MT Borchers NKG2D is Required for Regulation of Lung Pathology and Dendritic Cell Function Following RSV Infection J. Infect. Dis., 2018-10-20;0(0):. 2018-10-20 [PMID: 29554301] (ICC, Mouse)
• Annette Fink, Angeliqué Renzaho, Matthias J. Reddehase, Niels A. W. Lemmermann The p36 Isoform of Murine Cytomegalovirus m152 Protein Suffices for Mediating Innate and Adaptive Immune Evasion Viruses 2013-12-16 [PMID: 24351798]
• Kroesen M, Nierkens S, Ansems M, Wassink M, Orentas R, Boon L, den Brok M, Hoogerbrugge P, Adema G A transplantable TH-MYCN transgenic tumor model in C57Bl/6 mice for preclinical immunological studies in neuroblastoma. Int J Cancer, 2013-09-14;134(6):1335-45. 2013-09-14 [PMID: 24038106] (Flow Cytometry, Mouse)
• Spear P, Barber A, Rynda-Apple A, Sentman C NKG2D CAR T-cell therapy inhibits the growth of NKG2D ligand heterogeneous tumors. Immunol Cell Biol, 2013-04-30;91(6):435-40. 2013-04-30 [PMID: 23628805] (CAR-T (Flow Cytometry), Mouse)
• Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells. J. Immunol., 2012-06-29;189(3):1162-72. 2012-06-29 [PMID: 22753933] (Flow Cytometry, Mouse)
• Cédric Coulouarn, Valentina M. Factor, Elizabeth A. Conner, Snorri S. Thorgeirsson Genomic modeling of tumor onset and progression in a mouse model of aggressive human liver cancer Carcinogenesis 2011-10-01 [PMID: 21771728] (Flow Cytometry, Mouse, Transgenic Mouse)
• Rivas MN, Hazzan M, Weatherly K, Gaudray F, Salmon I, Braun MY NK cell regulation of CD4 T cell-mediated graft-versus-host disease. J. Immunol., 2010-05-19;184(12):6790-8. 2010-05-19 [PMID: 20488796] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Raet1a
• Uniprot:
  • Q9JI58()