Rad23 Antibody (3C12) - Azide and BSA Free

Western Blot: Rad23 Antibody (3C12) [H00005886-M01] - Analysis of RAD23A over-expressed 293 cell line, cotransfected with RAD23A Validated Chimera RNAi ( Cat # H00005886-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD23A monoclonal antibody (M01), clone 3C12 (Cat # H00005886-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: Rad23 Antibody (3C12) [H00005886-M01] - Analysis of RAD23A expression in transfected 293T cell line by RAD23A monoclonal antibody (M01), clone 3C12. Lane 1: RAD23A transfected lysate (39.6 KDa). Lane 2: Non-transfected lysate.

Sandwich ELISA: Rad23 Antibody (3C12) [H00005886-M01] - Detection limit for recombinant GST tagged RAD23A is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 3C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Rad23 Antibody (3C12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RAD23A (AAH14026, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEHGSVQESQVSEQPATEAAGENPLEFLRDQPQFQNMRQVIQQ
• Specificity: RAD23A - RAD23 homolog A (S. cerevisiae)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD23A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rad23 Antibody (3C12) - Azide and BSA Free

• hHR23
• hHR23A
• HR23A
• Rad23

Background

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rad23 Antibody (H00005886-M01)(4)

Publications using H00005886-M01

• Bang-Hung L, Tein-Ming Y, Chih-Jou H et al. DNA repair proteins as the targets for paroxetine to induce cytotoxicity in gastric cancer cell AGS. Am J Cancer Res . 2022-04-15 [PMID: 35530295]
• Tan X, Wang HC, Liang RY, Chuang SM. Human Rad23A plays a regulatory role in autophagy. Biochem Biophys Res Commun 2016-09-30 [PMID: 27613096]
• Tana X, Lianga RY, Chuang SM. hHR23A is required to control the basal turnover of Chk1. Cell Signal. 2015-08-18 [PMID: 26296656]
• Shen YH, Chen BR, Cherng SH et al. Cisplatin transiently up-regulates hHR23 expression through enhanced translational efficiency in A549 adenocarcinoma cells. Toxicol Lett. 2011-07-02 [PMID: 21742020]

Bioinformatics

• Gene Symbol: RAD23A
• Entrez:
  • 5886(Human)
• OMIM:
  • 600061(Human)
• Uniprot:
  • AAH14026(Human)