RAD18 Antibody (SN66-01)

Western Blot: RAD18 Antibody (SN66-01) [NBP2-67669] - Analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T Lane 3: MCF-7

Immunocytochemistry/Immunofluorescence: RAD18 Antibody (SN66-01) [NBP2-67669] - Staining RAD18 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RAD18 Antibody (SN66-01) [NBP2-67669] - Analysis of paraffin-embedded rat testis tissue using anti-RAD18 antibody. Counter stained with hematoxylin.

Flow Cytometry: RAD18 Antibody (SN66-01) [NBP2-67669] - Analysis of Hela cells with RAD18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunocytochemistry/Immunofluorescence: RAD18 Antibody (SN66-01) [NBP2-67669] - Staining RAD18 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: RAD18 Antibody (SN66-01) [NBP2-67669] - Staining RAD18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

RAD18 Antibody (SN66-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human RAD18 aa 50-99 / 495. (SwissProt: Q9NS91 Human; SwissProt: Q9QXK2 Mouse; Unigene: 22793 Rat)
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RAD18
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RAD18 Antibody (SN66-01)

• EC 6.3.2.-
• hHR18
• hRAD18
• postreplication repair protein hRAD18p
• Postreplication repair protein RAD18
• RAD18 homolog (S. cerevisiae)
• RAD18, S. cerevisiae, homolog
• RING finger protein 73
• RNF73E3 ubiquitin-protein ligase RAD18

Background

RAD18 is encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD18