Rac1,2,3 Antibody (AT2G10) - BSA Free

Western Blot: Antibody (AT2G10) [NBP2-88839] - The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. Jurkat cell lysate

Flow Cytometry: Antibody (AT2G10) [NBP2-88839] - Flow cytometry analysis of Rac1,2,3 in Jurkat cells. The cell was stained with at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Western Blot: Antibody (AT2G10) [NBP2-88839] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Rac1,2,3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human Rac 1 proteinLane 2.: Recombinant human Rac 2 proteinLane 3.: Recombinant human Rac 3 protein

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: AT2G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Rac1,2,3 Antibody (AT2G10) - BSA Free Summary

• Description: Novus Biologicals Mouse Rac1,2,3 Antibody (AT2G10) - BSA Free (NBP2-88839) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Rac1,2,3 (1-189aa) protein purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 2-5ug
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Rac1,2,3 Antibody (AT2G10) - BSA Free

• EN-7
• Gx
• HSPC022
• MIG5
• p21-Rac1
• p21-Rac2
• p21-Rac3
• Rac-1
• ras-related C3 botulinum toxin substrate 3
• rho family, small GTP binding protein Rac3
• TC-25

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAC1