RAB6B Antibody (6D12E4) - BSA Free

Western Blot: RAB6B Antibody (6D12E4) [NBP2-61825] - Analysis using Rab6b mAb against HEK293 (1) and Rab6b (AA: 95-208)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: RAB6B Antibody (6D12E4) [NBP2-61825] - Analysis of HepG2 cells using Rab6b mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: RAB6B Antibody (6D12E4) [NBP2-61825] - Analysis of paraffin-embedded ovarian cancer tissues using Rab6b mouse mAb with DAB staining.

Flow Cytometry: RAB6B Antibody (6D12E4) [NBP2-61825] - Analysis of Hela cells using Rab6b mouse mAb (green) and negative control (red).

Western Blot: RAB6B Antibody (6D12E4) [NBP2-61825] - Analysis using Rab6b mAb against human Rab6b (AA: 95-208) recombinant protein. (Expected MW is 38.7 kDa)

Western Blot: RAB6B Antibody (6D12E4) [NBP2-61825] - Analysis using Rab6b mouse mAb against C6 (1), HT-29 (2), and PC-12 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: RAB6B Antibody (6D12E4) [NBP2-61825] - Analysis of Hela cells using Rab6b mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: RAB6B Antibody (6D12E4) [NBP2-61825] - Analysis of paraffin-embedded lung cancer tissues using Rab6b mouse mAb with DAB staining.

ELISA: RAB6B Antibody (6D12E4) [NBP2-61825] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 6D12E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RAB6B Antibody (6D12E4) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human RAB6B (AA: 95-208) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: Rab6b
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 23.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for RAB6B Antibody (6D12E4) - BSA Free

• RAB6B, member RAS oncogene family
• ras-related protein Rab-6B
• small GTPase RAB6B
• small GTP-binding protein

Background

RAB6B (RAB6B, Member RAS Oncogene Family) is a Protein Coding gene. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics. GO annotations related to this gene include GTP binding and GTPase activity. An important paralog of this gene is RAB41.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Rab6b