RAB3IP Antibody - Azide and BSA Free

Western Blot: RAB3IP Antibody [H00117177-B01P] - Analysis of RAB3IP expression in rat brain.

Chromatin Immunoprecipitation: RAB3IP Antibody [H00117177-B01P] - 5dpf neuromast immunostained for Rab3IP (red) and counterstained with phalloidin (green). Image courtesy of customer.

Western Blot: RAB3IP Antibody [H00117177-B01P] - Analysis of RAB3IP expression in human placenta.

Western Blot: RAB3IP Antibody [H00117177-B01P] - Analysis of RAB3IP expression in transfected 293T cell line by RAB3IP polyclonal antibody. Lane 1: RAB3IP transfected lysate(50.6 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Rt, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RAB3IP Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse RAB3IP Antibody - Azide and BSA Free (H00117177-B01P) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-RAB3IP Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: RAB3IP (NP_071901.2, 1 a.a. - 460 a.a.) full-length human protein. MANDPLEGFHEVNLASPTSPDLLGVYESGTQEQTTSPSVIYRPHPSALSSVPIQANALDVSELPTQPVYSSPRRLNCAEISSISFHVTDPAPCSTSGVTAGLTKLTTRKDNYNAEREFLQGATITEACDGSDDIFGLSTDSLSRLRSPSVLEVREKGYERLKEELAKAQRELKLKDEECERLSKVRDQLGQELEELTASLFEEAHKMVREANIKQATAEKQLKEAQGKIDVLQAEVAALKTLVLSSSPTSPTQEPLPGGKTPFKKGHTRNKSTSSAMSGSHQDLSVIQPIVKDCKEADLSLYNEFRLWKDEPTMDRTCPFLDKIYQEDIFPCLTFSKSELASAVLEAVENNTLSIEPVGLQPIRFVKASAVECGGPKKCALTGQSKSCKHRIKLGDSSNYYYISPFCRYRITSVCNFFTYIRYIQQGLVKQQDVDQMFWEVMQLRKEMSLAKLGYFKEEL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: RAB3IP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This antibody is useful for Western Blot, Functional. Use in ICC/IF was reported in scientific literature (PMID: 28883094).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RAB3IP Antibody - Azide and BSA Free

• FLJ14660
• FLJ22548
• MGC71495
• RAB3A interacting protein (rabin3)
• rab-3A-interacting protein
• Rab3A-interacting protein
• RABIN3
• rabin-3
• SSX2 interacting protein
• SSX2-interacting protein

Background

RAB3IP, also known as Rab-3A-interacting protein, is a 53 kDa 476 amino acid protein with 8 isoforms produced by alternative splicing. The primary function of RAB3IP is to change GDP proteins into GTP proteins. Current research on RAB3IP is being conducted in relation to vitelliform, macular dystrophy, pilocytic astrocytoma, anaplastic astrocytoma and astrocytoma. RAB3IP is linked to the vesicle docking, metaphase, exocytosis, mitosis, transport, synaptic transmission and cytokinesis pathways where it interacts with RAB3IL1, SSX2B, SSX2, RAB8A and RAB3A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAB3IP Antibody (H00117177-B01P)(2)

Publications using H00117177-B01P

• Goodman L, Zallocchi M Integrin alpha 8 and Pcdh15 act as a complex to regulate cilia biogenesis in sensory cells J Cell Sci. 2017-10-31 [PMID: 28883094] (ICC/IF, Zebrafish)
• Sakamori R, Das S, Yu S et al. Cdc42 and Rab8a are critical for intestinal stem cell division, survival, and differentiation in mice. J Clin Invest. 2012-02-22 [PMID: 22354172]

Review for RAB3IP Antibody (H00117177-B01P) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Zebrafish.

reviewed by: Verified Customer IF Zebrafish 02/28/2017

Summary

• Application: Immunofluorescence
• Sample Tested: whole mount zebrafish
• Species: Zebrafish

Comments

• Comments: Whole 3-5dpf fish was fixed at 4⁰C for 4 hours with 4% PFA, 4% sucrose and 0.01% Tween 20 in PBS and then rinsed twice with PBSDT. Primary antibody was diluted in PBSDT containing 3% BSA and 6% FBS at 1:200 dilution and samples incubated overnight with rocking at 4⁰C. After several washes larvae were incubated with AlexaFluor 594-mouse conjugated antibody and AlexaFluor 488-phalloidin

Bioinformatics

• Gene Symbol: RAB3IP
• Uniprot:
  • NP_071901.2(Human)