RAB3IP Antibody (OTI5F2)

Western Blot: RAB3IP Antibody (OTI5F2) [NBP2-45500] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB3IP .

Immunocytochemistry/Immunofluorescence: RAB3IP Antibody (OTI5F2) [NBP2-45500] - RAB3IP Antibody (5F2) [NBP2-45500] - 3dpf zebrafish neuromast immunostained for Rab3IP (red) and counterstained with phalloidin (green). Image courtesy of customer.

Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

Immunohistochemistry-Paraffin: RAB3IP Antibody (OTI5F2) [NBP2-45500] - Staining of paraffin-embedded Human colon tissue within the normal limits using anti-RAB3IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3min) (1:150)

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, ICC/IF, IHC
• Clone: OTI5F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RAB3IP Antibody (OTI5F2) Summary

• Immunogen: Full length human recombinant protein of human RAB3IP (NP_071901) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAB3IP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 51.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RAB3IP Antibody (OTI5F2)

• FLJ14660
• FLJ22548
• MGC71495
• RAB3A interacting protein (rabin3)
• rab-3A-interacting protein
• Rab3A-interacting protein
• RABIN3
• rabin-3
• SSX2 interacting protein
• SSX2-interacting protein

Background

RAB3IP, also known as Rab-3A-interacting protein, is a 53 kDa 476 amino acid protein with 8 isoforms produced by alternative splicing. The primary function of RAB3IP is to change GDP proteins into GTP proteins. Current research on RAB3IP is being conducted in relation to vitelliform, macular dystrophy, pilocytic astrocytoma, anaplastic astrocytoma and astrocytoma. RAB3IP is linked to the vesicle docking, metaphase, exocytosis, mitosis, transport, synaptic transmission and cytokinesis pathways where it interacts with RAB3IL1, SSX2B, SSX2, RAB8A and RAB3A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for RAB3IP Antibody (NBP2-45500) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Zebrafish.

reviewed by: Verified Customer IF Zebrafish 02/28/2017

Summary

• Application: Immunofluorescence
• Sample Tested: whole mount zebrafish
• Species: Zebrafish

Comments

• Comments: 3-5dpf larvae were fixed at 4⁰C overnight with 4% PFA in PBS and then rinsed twice with PBS + Triton X-100 0.1%. Primary antibody was diluted in PBSTx 0.1% containing 2.5% BSA and 2.5% FBS at 1:200 dilution and samples incubated overnight with rocking at 4⁰C. After several washes larvae were incubated with AlexaFluor 594-mouse conjugated antibody and AlexaFluor 488-phalloidin.

Bioinformatics

• Gene Symbol: RAB3IP