RAB34 Overexpression Lysate (Native)

Images

 
Western Blot: RAB34 Overexpression Lysate (Adult Normal) [NBL1-15063] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAB34.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RAB34 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209722 and based on accession number NM_031934. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens RAB34, member RAS oncogene family (RAB34), transcript variant 1, mRNA.
Gene
RAB34

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for RAB34

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RAB34 Overexpression Lysate (Native)

  • RAB34, member RAS oncogene family
  • RAHRAB39Ras-related protein Rab-39
  • ras-related protein Rab-34
  • Ras-related protein Rah

Background

RAB proteins, like RAB34, are small GTPases that regulate vesicle budding, docking, and fusion along endocytosis and exocytosis pathways (Chen et al., 2003 [PubMed 12684051]).[supplied by OMIM]. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-13193
Species: Hu
Applications: IHC, IHC-P
NBP1-05048
Species: Hu, Mu, Rt, Bv, Ca, Sh
Applications: WB, ICC/IF
H00338382-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC-P, IP
NBP1-83967
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-91991
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-684
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
NB120-13253
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
MAB6188
Species: Hu
Applications: IHC
NBP2-32449
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-33110
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-81804
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
NB100-56106
Species: Hu, Mu, Rt, Ca, Ge
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
AF1029
Species: Mu
Applications: WB, IHC, IP
NB100-91266
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, ICC
NBP1-85968
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-44501
Species: Hu, Mu, Rt
Applications: Flow, IHC-P, IF

Publications for RAB34 Lysate (NBL1-15063) (0)

There are no publications for RAB34 Lysate (NBL1-15063).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RAB34 Lysate (NBL1-15063) (0)

There are no reviews for RAB34 Lysate (NBL1-15063). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RAB34 Lysate (NBL1-15063) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional RAB34 Products

Bioinformatics Tool for RAB34 Lysate (NBL1-15063)

Discover related pathways, diseases and genes to RAB34 Lysate (NBL1-15063). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RAB34 Lysate (NBL1-15063)

Discover more about diseases related to RAB34 Lysate (NBL1-15063).
 

Pathways for RAB34 Lysate (NBL1-15063)

View related products by pathway.

PTMs for RAB34 Lysate (NBL1-15063)

Learn more about PTMs related to RAB34 Lysate (NBL1-15063).
 

Research Areas for RAB34 Lysate (NBL1-15063)

Find related products by research area.

Blogs on RAB34

There are no specific blogs for RAB34, but you can read our latest blog posts.

Customers Who Bought This Also Bought

RAB34 Antibody
NBP1-87177
BID Antibody
NB100-56106

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RAB34 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol RAB34