Rab27a Overexpression Lysate (Native)


Western Blot: RAB27A Overexpression Lysate (Adult Normal) [NBP2-04777] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAB27A.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

Rab27a Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_899057. The protein contains a C-terminal DDK tag.
Homo sapiens RAB27A, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
24.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Rab27a

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Rab27a Overexpression Lysate (Native)

  • GS2
  • GS2rab-27
  • GTP-binding protein Ram
  • HsT18676
  • Rab-27
  • RAB27A, member RAS oncogene family
  • RAB27MGC117246
  • RAM
  • RAMras-related protein Rab-27A


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
Species: Mu
Applications: WB, ICC/IF, IHC-P, PEP-ELISA
Species: Hu
Applications: WB, ICC/IF, PEP-ELISA
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, PEP-ELISA
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Ze
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
Applications: WB

Publications for Rab27a Lysate (NBP2-04777) (0)

There are no publications for Rab27a Lysate (NBP2-04777).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Rab27a Lysate (NBP2-04777) (0)

There are no reviews for Rab27a Lysate (NBP2-04777). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Rab27a Lysate (NBP2-04777) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Rab27a Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for Rab27a Lysate (NBP2-04777)

Discover related pathways, diseases and genes to Rab27a Lysate (NBP2-04777). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Rab27a Lysate (NBP2-04777)

Discover more about diseases related to Rab27a Lysate (NBP2-04777).

Pathways for Rab27a Lysate (NBP2-04777)

View related products by pathway.

PTMs for Rab27a Lysate (NBP2-04777)

Learn more about PTMs related to Rab27a Lysate (NBP2-04777).

Research Areas for Rab27a Lysate (NBP2-04777)

Find related products by research area.

Blogs on Rab27a

There are no specific blogs for Rab27a, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol RAB27A

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-04777 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

Rab27a Antibody (1G7)
Rab27a Antibody (1G7)
Rab27a Antibody (1G7)
RAB8A Antibody (3G1)
RAB8A Antibody (3G1)
RAB8A Antibody (3G1)