Rab27a Antibody (2537A) [Alexa Fluor® 700]

• Applications: WB, ICC/IF
• Clone: 2537A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 700

Rab27a Antibody (2537A) [Alexa Fluor® 700] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant human RAB27a
  Ser135-Ala218
  Accession # P51159
• Specificity: Detects human RAB27a in direct ELISAs.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

RAB27A (Ras-related protein Rab 27A; also GTP-binding protein Ram) is a 27-28 kDa member of the Rab27 subfamily, Rab family, Small GTPase superfamily of proteins. It is widely expressed, and found in cells diverse as mast cells, cytotoxic T cells, melanocytes, retinal pigment epithelium and pancreatic beta -cells. RAB27A plays a key role in the secretion of specialized lysosomes termed secretory lysosomes. In melanocytes, for example, RAB27A is incorporated into the melanosome membrane where it serves as a docking factor for melanophilin and myosin-Va, regulating melanosome transport to, and concentration at, sites of release. Human RAB27A is 221 amino acids (aa) in length. It contains multiple Rab family and subfamily motifs, and concludes with a C-terminal CXC prenylation sequence (aa 219‑221). There is one potential splice variant that shows a deletion of aa 146-153. Over aa 135-218, human RAB27A shares 92% and 94% aa sequence identity with mouse Rab27A and rat RAB27A, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.